2 - TI Pharma

Chapter 8 Glucocorticoids affect intermediary metabolism
SUPPLEMENTARy MATERIAL
Supplementary Figure 1. Study Design (a) and overview of the 2-step hyperinsulinemic-euglycemic
clamp protocol (b).
Day -14 -2 -1 0
7
13 14
S T1 T2 M
V T1 T2
170
a
Baseline
PLB, PRED 7.5 mg or PRED 30 mg QD
Offtreatment
S: Screening T1: MRI/DEXA scan T2: Clamp M: start study medication V: Follow-up visits
b
Insulin 60 mU/m2 Insulin 20 mU/m .min
Glucose 5.0 mM
2 .min
Variable Glucose 20% enriched with 6,6 –2H 2 Glucose
6,6 –2H 2 Glucose bolus + Continuous infusion
1-13C 1 -Valine bolus + continuous infusion
1,1,2,3,3-2H 5 -Glycerol bolus + continuous infusion
0 120 Time (min)
240
360
Supplementary Figure 2. Patient flow diagram. 100% of the randomised participants completed the
study
28
V