EURON and THEME joint PhD meeting

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84 EURON and THEME joint meeting 2011 Effects of anticonvulsant drugs on hippocampal inhibitory microcircuits in the epileptic hippocampus Leonie Pothmann, Heinz Beck Department of Epileptology and Life&Brain Center, University Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany. The anticonvulsant carbamazepine (CBZ) acts via inhibition of voltage-gated Na + channels, showing an increase of inhibition when neurons fire at high frequencies. This use-dependent block is thought to be important in mediating the inhibition of pathological high-frequency seizure activity. However, some inhibitory interneuron types are capable of firing at very high rates, suggesting that CBZ should cause impaired GABAergic inhibition, and consequently increase excitability. Therefore, we examined the effects of CBZ on different cell types in the rat hippocampus. We found that pyramidal cell firing was inhibited by CBZ, as expected. However, interneurons that predominantly target pyramidal cell somata (group I), and interneurons targeting the apical dendritic tuft of pyramidal neurons (group II) did not show significant effects of CBZ. A third group of interneurons innervating the proximal dendrites of pyramidal cells in stratum oriens and stratum radiatum was affected by CBZ, showing a decrease of 44 % in maximal firing rate. To determine the impact of this reduction on inhibitory micronetworks we recruited feed-back and feed-forward inhibition of CA1 pyramidal cells by electrical stimulation of CA1 and CA3 pyramidal cell axons, respectively. At stimulation frequencies of 50 Hz, neither feed-back nor feed-forward inhibition was disturbed. In the pilocarpine model of chronic epilepsy we found marked changes in the responsiveness of different cell types: pyramidal cells showed a significant decrease of 14 % in the response to CBZ, whereas group I interneurons that where unresponsive in control animals now showed a block of 40 % in maximal firing rate. In contrast the pronounced effect on group III interneurons was lost in epileptic animal whereas the resistance of group II interneurons was unchanged. These data suggest differences in the properties of Na+ channels of different cell types, and that epileptogenesis leads to a marked change in the network effects of anticonvulsant drugs.
85 EURON and THEME joint meeting 2011 Maternal fluoxetine exposure, regardless of prenatal stress, affects physiological systems involved in sexual development of offspring Ine Rayen 1 , Thierry D. Charlier 2 , Stephanie Mollegaard Kristensen 1 , Jacques Balthazart 2 , Harry Steinbusch 1 , Jodi L. Pawluski 1,2 1 School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 50, 6229 ER Maastricht, The Netherlands; 2 University of Liege, GIGA-Neurosciences, 1 avenue de l'Hôpital (Bat. B36), B-4000 Liège, Belgium. Depression during pregnancy and postpartum is a growing health problem and affects up to 20% of women. While selective serotonin reuptake inhibitor (SSRIs) medications are commonly used for treatment of maternal depression, the combined effect of maternal depression and perinatal SSRI exposure on offspring development is poorly investigated. Here, our aim was to determine the role of exposure to fluoxetine during development on sexual behavior in offspring using a rodent model of maternal adversity. To do this, gestationally stressed and nonstressed Sprague-Dawley rat dams were chronically treated throughout lactation with either fluoxetine (5mg/kg/day) or vehicle beginning on postnatal day 1 (P1). Four groups of male and female offspring were obtained: 1) prenatal stress + fluoxetine exposure, 2) prenatal stress + vehicle, 3) fluoxetine exposure alone, and 4) vehicle alone. In Experiment 1, we assessed ano-genital (AG) distance in juvenile male and female offspring. In Experiment 2, adult male and female offspring were tested to assess the effect of developmental exposure to fluoxetine and prenatal stress on sexual behavior. Maternal fluoxetine exposure significantly decreased AG distance in juvenile male offspring, regardless of prenatal stress. AG distance in juvenile female offspring was not affected. In adult male offspring, preliminary results demonstrate that maternal fluoxetine treatment, regardless of exposure to prenatal stress, significantly decreased the number of intromissions, significantly increased the latency to first intromission and tended to increase the latency to first ejaculation. Further work will investigate the effect of prenatal stress and developmental fluoxetine exposure on sexual behavior in adult female offspring. We will also investigate the neurobiological plasticity underlying these behavioral effects. These preliminary results provide important evidence of the long-term impact of maternal fluoxetine use on the development of fundamental physiological systems.
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EURON and THEME joint PhD meeting U
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The local organizing committee: Pro
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Information about THEME 3 EURON and
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5 EURON and THEME joint meeting 201
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EURON a short introduction 7 EURON
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Programme 13 EURON and THEME joint
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Thursday September 22 nd 9:00 - 10:
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Friday September 23 rd 17 EURON and
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Poster presentations 19 EURON and T
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EURON Lecture Frank Kirchhoff ADDRE
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ABSTRACT 25 EURON and THEME joint m
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THEME Lecture Eckhard Mandelkow POS
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ABSTRACT 29 EURON and THEME joint m
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Abstracts 31 EURON and THEME joint
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Page 105 and 106: List of Participants 103 EURON and
Page 107 and 108: Nuersailike Abuduwali University of
Page 109 and 110: Marianne Eisenhardt University of B
Page 111 and 112: Sabine Klein University of Bonn PhD
Page 113 and 114: Viktoriya Peeva University of Bonn
Page 115: Sylvie van der Kruijs Maastricht Un
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EURON and THEME joint PhD meeting

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