EURON and THEME joint PhD meeting

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70 EURON and THEME joint meeting 2011 area of corpus callosum around the midline. Moreover, the number of NG2 + oligodendrocyte progenitor cells (OPC) and Iba-1 + microglia were found to be significantly reduced compared to controls. In vitro, IL-11 dose dependently increased myelin phagocytosis (1, 3, 10, 30 ng/ml) by RAW 264.7 cells. Currently, the effect of therapeutic application of IL-11 is being evaluated. Conclusion Prophylactic delivery of IL-11 reduces acute demyelination in the cuprizone model. IL-11 potentiates myelin phagocytosis in vitro, which could point to enhanced clearing of myelin debris in vivo thereby creating an environment conducive for repair processes in the demyelinated area. Moreover, the reduced number of OPC may indicate that differentiation into mature myelinating oligodendrocytes has occurred, which then play their putative role in remyelination. Future studies will have to confirm these findings.
71 EURON and THEME joint meeting 2011 Identification of microRNAs regulating neuronal differentiation of human neural stem cells Laura Mürtz 1 , Lodovica Borghese 1 , Beate Roese-Koerner 1 , Sandra Weinhold 2 , Philipp Koch 1 , Peter Wernet 2 , Markus Uhrberg 2 and Oliver Brüstle 1 1 Institute of Reconstructive Neurobiology, Life and Brain Center, University of Bonn; 2 Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine University Düsseldorf. MicroRNAs are a class of small non-coding RNAs that act as post-transcriptional regulators of gene expression. Studies in model organisms have demonstrated critical roles for a number of microRNAs in neuronal development, but their specific functions have not been fully examined. Likewise, data on microRNA function in the context of human neural development and neural stem cell biology is particularly scarce. Our human embryonic stem cell (hESC) based neural differentiation paradigm provides a useful model for the analysis of microRNA profiles in human pluripotent and multipotent stem cells and for the identification and functional characterization of novel microRNAs associated with neuronal differentiation. We assessed microRNA of human ES cells (hES, I3 line), of long-term self-renewing neuroepithelial-like stem cells (lt-NES® cells) (Koch et al. 2009) and of 15- and 30-days old differentiated neuronal cultures (ND15, ND30) using a quantitative real-time PCR multiplex assay. We validated the identified expression patterns for several microRNAs by Northern blot analysis in two independent hES cell lines (I3 and H9.2) indicating the reliability of our approach. Furthermore we were able to confirm previous assignments of ES cell-specific and brain-specific microRNAs. In addition, we identified several novel microRNAs, besides the known neuronal microRNAs, i.e. miR-124 and miR-125, as up-regulated during human neural stem cell differentiation. Stable overexpression of the newly identified microRNAs, just like the overexpression of miR-124 and miR-125, resulted in an increased rate of neuronal differentiation. Our data demonstrate that these microRNAs are not only markers of differentiation but may also function as regulators of human neuronal differentiation.
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EURON and THEME joint PhD meeting U
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The local organizing committee: Pro
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Information about THEME 3 EURON and
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5 EURON and THEME joint meeting 201
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EURON a short introduction 7 EURON
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Programme 13 EURON and THEME joint
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Thursday September 22 nd 9:00 - 10:
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Friday September 23 rd 17 EURON and
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Page 25 and 26: EURON Lecture Frank Kirchhoff ADDRE
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Page 29 and 30: THEME Lecture Eckhard Mandelkow POS
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Page 33 and 34: Abstracts 31 EURON and THEME joint
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Page 105 and 106: List of Participants 103 EURON and
Page 107 and 108: Nuersailike Abuduwali University of
Page 109 and 110: Marianne Eisenhardt University of B
Page 111 and 112: Sabine Klein University of Bonn PhD
Page 113 and 114: Viktoriya Peeva University of Bonn
Page 115: Sylvie van der Kruijs Maastricht Un
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EURON and THEME joint PhD meeting

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