EURON and THEME joint PhD meeting

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48 EURON and THEME joint meeting 2011 see that numerous GO terms are down-regulated in the PA group. These data will be further analyzed and visualized in pathways; results are expected early September 2011. Conclusions These data in rodent fetuses and neonates are the first to demonstrate global gene expression profiles in the brain after fetal preconditioning and perinatal asphyxia. From previous behavioral studies we know that adult FA+PA animals perform similar to adult CCD animals despite the fact that the FA+PA group experienced a perinatal asphyctic insult. Interestingly here we also found no difference between these groups at 6hours after birth.
49 EURON and THEME joint meeting 2011 Labelling and optogenetic manipulation of learningrelated neuronal assemblies. Holger Dannenberg, Milan Pabst, Karen van Loo, Albert Becker, Susanne Schoch, Heinz Beck Department of Epileptology and Life&Brain Center, University Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany. Learning is thought to correlate with an initial change in the dynamics of a specific assembly of neurons and a subsequent sustained change in the synaptic connections between them. The propensity of this particular assembly of neurons to be recruited into synchronized activity can then be viewed as a memory trace. However, identifying these neuronal assemblies has been challenging. Immediate early gene promoters (IEGs) could be good candidates to genetically label cell populations active in-vitro, and to subsequently drive expression of fluorescent markers or light-sensitive channels that allow specific millisecondscale modulation of cellular activity. We have first compared the hippocampal expression patterns of the IEGs Arc (also known as Arg3.1), Egr1 (also known as zif268) and c-fos after a spatial learning paradigm in rats. We found an increase in the number of cells in the hippocampus of trained rats and/or rats having explored a novel environment compared to a cage control group. The expression patterns showed a partial overlap with subregional differences between each of the IEGs. We first chose a core promoter from the Arc gene (termed synaptic-activityresponsive element, SARE) to drive expression of the light-activated inhibitory chloride-pump halorhodopsin (eNpHR3.0) fused to EYFP. We show that the adenoviral approach is suitable to express eNpHR3.0-EYFP in principal cells of the hippocampus and characterized the SARE activation pattern by comparing it with the expression pattern of Arc, Egr1, and c-fos. eNpHR3.0-EYFP expressing cells could be inhibited by photo-activation in acute hippocampal slices, confirming its functionality in vitro. These approaches may permit the light-based activation or inhibition of neurons participating in the neuronal engram, both in-vitro and in-vivo.
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List of Participants 103 EURON and
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Nuersailike Abuduwali University of
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Marianne Eisenhardt University of B
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Sabine Klein University of Bonn PhD
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Viktoriya Peeva University of Bonn
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Sylvie van der Kruijs Maastricht Un
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EURON and THEME joint PhD meeting

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