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FSBI – 2004 Food Science and Biotechnology in Developing Countries Bleaching of Amaranth Oil (A. hypochondriacus) Ariza-Ortega J.A. (1) , López-Valdez F. (2) , Montalvo-Paquini C. (3) , Arellano-Huacuja A. (4) , and Luna- Suárez S. (5) . (1) CICATA-IPN Puebla. Acatlán 63, La Paz. Puebla, Pue. México 72160. E-mail: ariza_ortega@yahoo.com.<strong>mx</strong> (2) CICATA-IPN Puebla. Acatlán 63, La Paz. Puebla, Pue. México 72160. E-mail: flopez2072@yahoo.com (3) CICATA-IPN Puebla. Acatlán 63, La Paz. Puebla, Pue. México 72160. E-mail: cpaquini@yahoo.com (4) CICATA-IPN Puebla. Acatlán 63, La Paz. Puebla, Pue. México 72160. E-mail: amy@puebla.megared.net.<strong>mx</strong> (5) CICATA-IPN Puebla. Acatlán 63, La Paz. Puebla, Pue. México 72160. E-mail: silvials2004@yahoo.com.<strong>mx</strong> Tel.: 52 (222) 230 - 4459. Tel/Fax: 249 - 8540. Abstract The bleaching was made by two methods, using 2 clays: actisil (A), optimum (O) and activated charcoal (AC). Method 1: The oil was mixed with 10 % A + O and 0.5 % (w/v) AC at 20º increasing each 10º up to 120° C. Method 2: The oil was mixed with 2 % A + O and 0.2 % (w/v) AC at 20°, 70º, 100º, 110º and 120º C. The best result in the method 1 was 1.01 units of photometric color (PC) at 120º C. The best result in the method 2 was 3.25 units PC at 70º C and 3.1 units PC at 20º C. Key words: Oil, bleaching, clays and photometric color. Introduction The obtained oils from different sources (mainly seeds) are called crude oils (1). The oil has undesired compounds that make it undesired to use. To resolve this problem, the oil is refined (2 y 3). Andersen (1965) proposed the following methods: sedimentation, centrifugation, filtration, degumming, neutralization, bleaching and deodorization. The bleaching is a method used to diminish pigments (carotenoid, flavonoides and others) that provide a colour nonwished in crudes oils. The most common methods for bleaching are; chemical action, hidrogenation, neutralization, heat and adsorption. The purpose of this work was bleaching the neutralized oil from amaranth seed of A. hypochondriacus with the adsorption method. Materials and Methods Amaranth oil Neutralized amaranth oil from Hidalgo (Mexico) was used. Bleaching Two methods were used: 1) the oil was mixed with clays: 10 % (w/v) each one of actisil (A) + optimum (O) and 0.5 % (w/v) activated charcoal (AC). Both treatments were working at 20º C increasing 10º up to 120° C and were stirred for 10, 20 and 30 minutes, each one. Then, were filtered. 2) The oil was mixed with clays: 2 % (w/v) (each one) of A + O and 0.2 % (w/v) AC. Both treatments were working at 20º, 70º, 100º, 110º and 120° C each one. Then, were stirred for 10, 20 and 30 minutes and filtered. To diminish the photometric color (PC) in oil, were carry out three steps mixing clays and activated charcoal each one of anterior treatments. The PC was measured in a Spectronic Genesis 2 spectrophotometer, using the method proposed by Allen (1982).