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Joint International Conference on Long-term Experiments ...

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A problem c<strong>on</strong>cerning the formati<strong>on</strong> of nitrosamines in meat spice-cure mixtures has<br />

recently been investigated. Salts, including nitrite, are added to a variety of spices in the<br />

formulati<strong>on</strong> of meat spice-cure mixtures. Amines present in spices could react with the<br />

added nitrite and form nitrosamines. It was c<strong>on</strong>firmed DMNA, N-nitrosopyrrolidine, and Nnitrosopiperidine<br />

in meat curing mixtures at levels up to 25 ppm.<br />

Until recently spice-cure mixtures have been marketed in several forms and formulati<strong>on</strong>s,<br />

such as single packaging of spices and curing salts, "piggy-back" (separate packaging of<br />

spices and curing salts), with and without sodium carb<strong>on</strong>ate buffers, and with and without<br />

protective coating of the nitrite crystals (zein corn protein).<br />

A survey of 32 spice-cure mixtures was undertaken to de<strong>term</strong>ine the effectiveness of<br />

the 3 marketing processes and also to de<strong>term</strong>ine the effect of storage time and temperature<br />

<strong>on</strong> nitros-amine formati<strong>on</strong>. The 9 samples found to c<strong>on</strong>tain nitrosamines are shown in Table<br />

4. All data in the table were c<strong>on</strong>firmed by MS.<br />

Three samples were part of a time-temperature stability study. Each was buffered with<br />

sodium carb<strong>on</strong>ate. The spice-cure mixtures were analyzed at room temperature and 100°F at<br />

0, 30, and 60 days storage. Samples 1 and 2 in Table 4 were nitrosamine-free when first analyzed,<br />

but after heat and storage, both c<strong>on</strong>tained N-nitrosopiperidine from 150 to 420 ppb<br />

and N-nitrosopyrrolidine from 50 to 95 ppb. The third sample (a c<strong>on</strong>trol not shown in Table<br />

4) did not c<strong>on</strong>tain nitrite and, as expected, no nitrosamines were found.<br />

Table 3. Food products analyzed: no nitrosamines detected<br />

Sample No. of analyses Product<br />

Pork Products<br />

23<br />

24<br />

25<br />

26<br />

27<br />

28<br />

29<br />

30<br />

31<br />

32a<br />

33<br />

34<br />

2<br />

1<br />

1<br />

1<br />

5<br />

6<br />

2<br />

2<br />

1<br />

1<br />

1<br />

1<br />

pork liver (raw)<br />

pork liver (fried)<br />

Polish style sausage (raw)<br />

Polish style sausage (boiled)<br />

Baby Foods<br />

beans, potatoes, and ham<br />

casserole creamed potatoes and ham<br />

Variety Meats<br />

Crumbled bac<strong>on</strong><br />

crumbled ham<br />

bac<strong>on</strong> (imported canned; raw)<br />

bac<strong>on</strong> (imported canned; fried)<br />

beer sticks<br />

smoked pepper<strong>on</strong>i<br />

Total Diet Samples<br />

35 1 dairy products<br />

535

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