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Joint International Conference on Long-term Experiments ...

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hours of germinati<strong>on</strong>, we observed an increase of the PA in the extract obtained from<br />

the roots as compared with the same parameter de<strong>term</strong>ined in the c<strong>on</strong>trol sample, the<br />

increase being very significant in the case of the germinati<strong>on</strong> in SA soluti<strong>on</strong> of 1.0mg/l,<br />

and significant when using a SA soluti<strong>on</strong> of 0.1mg/l c<strong>on</strong>centrati<strong>on</strong> (table 1).<br />

Studying the variati<strong>on</strong> of the same parameter after 96 hours, an insignificant<br />

increase of the peroxidase activity was observed as compared with the c<strong>on</strong>trol sample at<br />

c<strong>on</strong>centrati<strong>on</strong>s of 0.1 and 1.0 mg/l SA soluti<strong>on</strong> (table 1).<br />

After 120 hour of germinati<strong>on</strong>, we observed a very signifiant increase of the PA in<br />

the extract of the roots in the case of germinati<strong>on</strong> in 1.0mg/l SA soluti<strong>on</strong>, and an<br />

insignifiant increase for 0.1mg/l SA soluti<strong>on</strong>. (table1.)<br />

For the coleoptiles extracts, the intensity of the peroxidase activity after 72, 96 and<br />

120 hours of germinati<strong>on</strong> has insignificantly modified as compared with the same<br />

parameter of c<strong>on</strong>trol sample when we used 0.1mg/l SA soluti<strong>on</strong>, and significantly<br />

increase for 1mg/l SA soluti<strong>on</strong> for 72 and 120 hour of germinati<strong>on</strong> (table 1).<br />

4./ CONCLUSIONS<br />

It can be observed that the exogenous SA inhibits the germinati<strong>on</strong> energy of the maize<br />

caryopsis with 10 and 45% from c<strong>on</strong>trol lot, using SA soluti<strong>on</strong> with 0.1 and 1.0 mg/l<br />

c<strong>on</strong>centrati<strong>on</strong> and 100% in case of 5.0mg/l SA soluti<strong>on</strong>.<br />

The peroxidase activity of the vegetal extracts obtained from the roots of the<br />

plantlets obtained after germinati<strong>on</strong> <strong>on</strong> filter paper moistened with SA soluti<strong>on</strong> was<br />

very significantly increase after 72 and 120 hours from germinati<strong>on</strong> for 1.0 mg/l<br />

c<strong>on</strong>centrati<strong>on</strong> SA soluti<strong>on</strong> and significantly increase for 0.1mg/l SA soluti<strong>on</strong>, as<br />

opposed to the peroxidase activity of the extracts obtained from coleoptiles, where its<br />

activity changed insignificantly after 72 and 120 hours of germinati<strong>on</strong> for 0.1mg/l SA<br />

soluti<strong>on</strong>, and significantly for 1.0mg/l SA soluti<strong>on</strong>.<br />

In c<strong>on</strong>clusi<strong>on</strong> exogenous salicylic acid are influenced different physiological<br />

plant process.<br />

REFERENCES<br />

Andrei M., Anghel I., Popescu I., Stoica E., (1981): Lucrări practice de biologie<br />

vegetală, Editura didactică şi pedagogică Bucureşti.<br />

Antofie M., Carasan ME., Brezeanu A., (2003): Salicylic acid effects <strong>on</strong> two<br />

Gardenia Jasminoides cell lines. Acta Horti Bot. Bucureşti, 30:135-145.<br />

Barkosky RR., Einhellig FA., (1993): Effects of salicylic acid <strong>on</strong> plant water<br />

relati<strong>on</strong>ship. Journal of Chemical Ecolology, 19:237-247.<br />

Cleland FC., Ajami A., (1974) – Identificati<strong>on</strong> of the flower-inducing factor isolated<br />

from aphid h<strong>on</strong>ey dew as being salicylic acid. Plant Physiol. 54:904-906.<br />

Cutt JR. Klessig DF., (1992): Salicylic acid in plants. A changing perspective.<br />

Pharmacetical Technology, 16:25-34.<br />

Handro W., Mello CM., Manzano MA., Floh ES.,(1997): Promotive effects of<br />

Salicylic acid. R. Bras. Fisiol.Veg. 9(2): 139-142.<br />

Hans-Luck, (1970): Methdos of Enzymatic Analysis Verlag Chemie Gmb H.<br />

(Ed).Weinheim Bergstr. Academic Press, New york and L<strong>on</strong>d<strong>on</strong>.p.885-888.<br />

490

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