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Joint International Conference on Long-term Experiments ...

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was the explant type (the developmental stage of the zygotic embryo used as<br />

explant); the early developmental stages of the zygotic embryos 1 and 2 had a<br />

significant positive effect up<strong>on</strong> the embryo inducti<strong>on</strong> efficiency, but the more<br />

advanced stages (3 and 4) had a negative effect, comparing to the average.<br />

4. We can c<strong>on</strong>clude that it is very important to harvest the acorns for somatic<br />

embryogenesis experiments in early stages, and when it is not possible, to plant <strong>on</strong><br />

culture medium a big number of explants (5 fold more than usually), in order to<br />

ensure the somatic embryo inducti<strong>on</strong>, despite of the very low efficiency.<br />

5. The sec<strong>on</strong>d factor affecting the embryogenic ability, as importance, was the<br />

genotype. The best embryogenic ability was found in Q. petraea and the worst, in<br />

Q. frainetto. The embryogenic ability of Q. robur (average, 17,8%) and Q. petraea<br />

(av. 18,5%) was similar, but that of Q. frainetto was significantly inferior (average<br />

10,4%).<br />

6. C<strong>on</strong>cerning provenances, in Q. robur, the best results were obtained in the<br />

provenance Tg. Mures (av. 22,9%) and the worst, in Dumbrava Sibiului (av. 12,9).<br />

Between the Q. frainetto provenances, Griva was superior (av. 12,5%), and<br />

Padurea sarului was the worst (average 8,3%).<br />

7. The effect of culture medium proved to be totally insignificant, besides the<br />

differences observed am<strong>on</strong>g the variants cultivated <strong>on</strong> different media. The<br />

situati<strong>on</strong> was the same as well for the nutrients as for growth regulators<br />

8. The compositi<strong>on</strong> of culture medium was not a limitative factor, media with<br />

different nutritive compositi<strong>on</strong>s and auxin/cytokinin ratios of 5/1 (QE1, QE3) and<br />

1/1 (QE2, QE4) being equally suitable for the inducti<strong>on</strong> of somatic embryogenesis.<br />

BIBLIOGRAPHY<br />

1. Ardelean, M., Sestraş, R., Cordea, M., 2005 - “Tehnică experimentală horticolă”,<br />

Ediţie revizuită şi adăugită - Editura Academicpres, Cluj-Napoca, pg.18<br />

2. Chalupa, A.V., 1987 - "Somatic embryogenesis and plant regenerati<strong>on</strong> in<br />

Picea,Quercus, Betula, Tilia, Robinia, Fagus and Aesculus" - Comm. Inst. Forest.<br />

Cehosloveniae, Vol. 15: 133-148.<br />

3. Chalupa, A.V., 1990 - "Plant regenerati<strong>on</strong> by somatic embryogenesis from cultured<br />

immature embryos of oak (Quercus robur L.) and linden (Tilia cordata Mill.) -<br />

Plant cell. Rep., 9: 398-401<br />

4. Gupta, P.K., Durzan, D.J., 1985 - “Shoot multiplicati<strong>on</strong> from mature trees of<br />

Duglas fir (Pseudotsuga menziesii) and Sugar pine (Pinus lambertiana)”- Plant Cell<br />

Rep., 4: 177-179.<br />

5. Gingas,V.M, Lineberger, R.D., 1989 - "Asexual embryogenesis and plant<br />

regenerati<strong>on</strong> in Quercus" - Plant Cell, Tissue and Organ Culture, 17: 191-203.<br />

6. Jorgensen, J., 1988 - "Embryogenesis in Quercus petraea and Fagus sylvatica" - J.<br />

Plant physiol. 132: 638-640.<br />

7. Jorgensen, J., 1991 - "Cryopreservati<strong>on</strong> of haploid embryos of Quercus petraea,<br />

Fagus sylavtica and somatic embryos of Aesculus hippocastanum" - In: Ahuja<br />

(Ed.), Woody Plant Biotechnology, Plenum press: 355-356.<br />

8. Jorgensen, J., 1993 - "Embryogenesis in Quercus petraea" - Ann. Sci. For. 50,<br />

Suppl.1: 344s-350s.<br />

451

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