Joint International Conference on Long-term Experiments ...

Table 4 The effect of developmental stage of explant and culture medium upon the
rate of embryogenic induction in Q. robur (test Duncan)
Nr.
crt.
Stage of explants
(zygotic
embryos)
Culture medium
Rate of embryo induction(%) and significance*
Average
S1 S2 S3 S4
on
culture
media
1 QE1 0.76abc 0.65cde 0.57defg 0.45ghi 0.61 A
2 QE2 0.79ab 0.67cde 0.51fghi 0.43i 0.60 A
3 QE3 0.82a 0.61def 0.56efghi 0.44hi 0.61 A
4 QE4 0.83a 0.69bcd 0.59def 0.46ghi 0,64 A
Average on stages 0.80M 0.65N 0.56NP 0.44P
*Differences among any variants followed by one common letter are not significant.
DS 5% for the interaction between culture medium and stage: 0,106-0,129.
DS 5% for culture medium: 0,184-0,200.
DS 5% for the stage of explants: 0,134-0,145.
In the table 4 it is shown that the best results were obtained in the stage 1, on the media
QE3 and QE4, and the worst, in the stage 4 on the medium QE 2.
2.1.b. The effect of provenance, stage of explant and culture medium upon the embryo
induction efficiency in Quercus frainetto
The three way Anova for the effect of provenance, stage of explant and culture medium
upon the embryo induction efficiency in Q.s frainetto is presented in the Table 5.
Table 5 The three way Anova type 3x4x4 for the effect of (provenance x stage of
explant x culture medium) upon the embryo induction efficiency in Quercus frainetto
Source of variation MSD DF MS
F-test
TOTAL 2.524 95
Big plots 0.081 5
Replications 0.010 2
Factor A (PROVENANCE) 0.002 1 0.002 0,053.49; 5.95
Interaction A x B 0.045 3 0.015 1,95