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Joint International Conference on Long-term Experiments ...

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On sandy soil, the total N c<strong>on</strong>tent increased with increasing N doses, while adding of<br />

biofertilizer to soil caused no significant increase in soil CaCl2-soluble total nitrogen<br />

c<strong>on</strong>centrati<strong>on</strong> (Figure 2).<br />

15<br />

10<br />

5<br />

0<br />

Figure 2 Effect of N doses and biofertilizati<strong>on</strong> <strong>on</strong> 0.01 M CaCl2<br />

soluble total N c<strong>on</strong>tent <strong>on</strong> sandy soil (LSD5% (N): 3.9 LSD5% (B):<br />

3.37)<br />

Moreover, biofertilizer have a negative influence during the investigated period <strong>on</strong><br />

0.01M CaCl2-soluble total N c<strong>on</strong>tent of sandy soil with acidic character and low c<strong>on</strong>tent<br />

of organic matter.<br />

The nitrogen fertilizati<strong>on</strong>, beside the total N, enhanced the nitrate c<strong>on</strong>centrati<strong>on</strong>s in<br />

0.01 M CaCl2 extracti<strong>on</strong>s (Figure 3). Biofertilizer also have an increasing effect <strong>on</strong><br />

nitrate, but <strong>on</strong>ly at high doses of N.<br />

12<br />

10<br />

8<br />

6<br />

4<br />

2<br />

0<br />

B1 B2 B3<br />

N1 N2 N3 N4<br />

B1 B2 B3<br />

N1 N2 N3 N4<br />

Figure 3: Effect of nitrogen rates and biofertilizati<strong>on</strong> <strong>on</strong> nitrate<br />

c<strong>on</strong>centrati<strong>on</strong> <strong>on</strong> chernozem (LSD5% (N): 3.78 LSD5% (B): 3.28)<br />

317

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