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Joint International Conference on Long-term Experiments ...

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Table 5: N c<strong>on</strong>tent in plant <strong>on</strong> sandy soil<br />

Leaf N c<strong>on</strong>tent (%)<br />

Phylaz<strong>on</strong>it doses (cm 3 pot -1 N (g pot<br />

)<br />

-1 )<br />

80 40 0<br />

Mean LSD5% (N)<br />

0.2 3.2 2.8 2.8 2.9<br />

0.1 2.5 2.5 3.2 2.7<br />

0.05 2.1 2.4 2.0 2.2 0.4<br />

0 1.9 1.9 2.6 2.1<br />

Mean 2.4 2.4 2.6 2.5<br />

LSD5% (phylaz<strong>on</strong>it):0.3<br />

Between two soils there was a remarkable difference: in case of acidic sandy soil, the<br />

adding of biofertilizer caused an increase in both plant biomass and in N c<strong>on</strong>tent of leaf.<br />

These effects are not appeared for chernozem. Due to the very low N supply ability of<br />

sandy soil, the increment of microbes amount due to biofertilizati<strong>on</strong> may cause N<br />

immobilizati<strong>on</strong>, because the enhanced microbial biomass take up soluble N.<br />

To take further investigati<strong>on</strong> <strong>on</strong> this problem, 0.01 M CaCl2 soluble total N and nitrate<br />

was measured from soil samples taken at the end of growing period. Both N fertilizati<strong>on</strong><br />

and biofertilizati<strong>on</strong> significantly increased the soluble total nitrogen c<strong>on</strong>centrati<strong>on</strong> <strong>on</strong><br />

the chernozem soil (Figure 1).<br />

25<br />

20<br />

15<br />

10<br />

5<br />

0<br />

B1 B2 B3<br />

N1 N2 N3 N4<br />

Figure 1: Effect of N and biofertilizati<strong>on</strong> <strong>on</strong> 0.01 M CaCl2 soluble<br />

total N c<strong>on</strong>tent of chernozem (LSD5% (N): 2.90 LSD5% (B): 2.51)<br />

316

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