Joint International Conference on Long-term Experiments ...

PC 2
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4
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1
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-1
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V10
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V12 V13
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-6 -4 -2 0 2 4 6
PC 1
Figure 1: PCA biplot of the two species based on all variables.
(1-8, 14 vegetative, 9-13 reproductive variables.) □: transplanted P. angustifolia, ○:
transplanted P. humilis, �: control P. angustifolia, �: control P. humilis. v1: height,
v2: panicle length, v3: panicle width, v4: flag leaf sheath length, v5: flag leaf blade
length, v6: ligule length of the flag leaves, v7: the widest width of leaves, v8: flag leaf
blade width, v9: spikelet length, v10: upper glume length, v11: lower glume length,
v12: palea length, v13: lemma length, v14: longest leaf blade lengths of lateral tillers.
There was no separation based on the features of reproductive organs (Fig. 1). However,
the individuals were concentrated into two, partly overlapping groups based on the
characteristics of their vegetative organs. The first is the group of transplanted
individuals which have larger vegetative organs (Fig. 1). The second is the group of
individuals with smaller organs which were collected from the original turf clones. An
explanation for this is that in the experimental environment the interspecific competition
was suppressed as seedlings of other species were eliminated and the transplanted plants
were able to spread and occupy all of the available area.
The PCA results separated the transplanted and control populations of P.
angustifolia into two groups but the transplanted and control populations of P. humilis
did not show such separation (Fig. 1). The quantitative morphological characteristics of
P. angustifolia responded more sensitively to the transplantation.
Neither species nor transplanted populations were separated based on the
reproductive characters. The two species did not show differences considering the
vegetative characteristics, but the transplanted and control individuals of P. angustifolia
separated slightly.
In the AFLP analysis three primer combinations were used and generated clear banding
patterns. A total of 338 DNA fragments were produced across 12 genotypes of four Poa
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