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The genotypes differentiated considering the degree of callus development already from
the end of first week. By the end of fourth week the 2107 breeding line showed the
strongest callus forming capacity, while cv. Janus was the weakest. Only cv. Hunor and
Erbi belongs to the same group, the others differ from each other significantly (table 2.).
Table 2. Effect of genotypes on callus induction and growth in the average of all
treatments.
Callus development on the end of
1 st 2 nd 3 rd 4 th
Genotypes
week
Graphis 2.33 g 3.21 f 4.08 g 4.42 f
Baccara 1.88 f 2.69 d 3.51 e 4.30 e
2107 1.85 f 2.84 e 3.76 f 4.65 g
Hanka 1.76 e 2.67 d 3.20 d 4.05 d
Hunor 1.00 a 1.72 a 2.58 a 3.60 b
Janus 1.32 d 1.85 b 2.51 a 2.97 a
M49060 1.23 c 2.12 c 2.87 c 3.77 c
Erbi 1.10 b 1.79 ab 2.78 b 3.63 b
Means within column signed by the same letter belong to the same homogeneous group.
Similarly to Gantotti and Kartha (1983), who could induce the callus cultures on each
part of plant, excluding the root tip, we also obtained callus culture on both type of
explants (table 3.). Because of significant interactions and since we found significant
differences between callus forming capacity of different type of explants, we continued
the statistical analysis as one-way ANOVA.
Table 3. Effect of explant type on callus forming capacity in the average of all
treatments.
Callus development on the end of
1 st 2 nd 3 rd 4 th
Explant type
week
Leaflets 1.55 a 2.49 a 3.35 b 4.21 b
Stem segments 1.58 a 2.25 a 3.00 a 3.67 a
Means within column signed by the same letter did not differ significantly
Callus development was better on the leaf explants so we analysed the effect of auxins
on callus development on leaf explants. Molnár (1993) found 2,4-D to be suitable for
callus induction, and we also could induce callus development by the use of 2,4-D,
although the degree of callus development was decreased by 2,4-D, especially in higher
concentrations, excluding cv. Hanka (table 4). Gantchev (1997) also tested different
concentrations of 2,4-D (2.0-32.0 mgl -1 ) on internodal stem segments of pea, and found
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