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In conclusion, the fungal charge of grains for food and fodder on all samples is enough
high to alarm us, indifferent of the method of analyze. We have to corroborate this data
with mycotoxins analyses to know if our food and fodder is dangerous or not.
In the next table (Table 3) we present the natural occurrence of mycotoxins in
grains analyzed by TLC coupled with densitometry.
As shown in Table 3, we analyzed 39 samples from different Romanian districts,
20 samples were found contaminated with mycotoxins. Four samples were
contaminated with ochratoxin A and the concentration was higher than legal European –
we notice that in rye and a sample of wheat for bakery, the values were very alarming,
over 20 µg/kg. In 11 samples was identified AB1, in 9 samples the amont of this was
higher than norms; concerning the total concentration of aflatoxins 10 sample this was
higher than legal European norms. The concentration of aflatoxins is very dangerous in
biscuits for kids.
The contamination range was: aflatoxin B1: 1.7 - 5.7 µg/kg, aflatoxin B2: 0.02 -2.8
µg/kg, aflatoxin G1: 1.1 – 5.7 µg/kg, aflatoxin G2: 0.12 – 1.8 µg/kg, total aflatoxins: 1.2
– 10.8 µg/kg and ochratoxin A: 4.4 - 30.0 µg/kg. The higher number of contamination
rate was in the case of wheat samples.
I n conclusion, we can notice that the fungal charge of grains for food and fodder
and in bakery products on all samples is enough high to alarm us, no matter of the
method of analyze. The mycotoxin analyses reveal to us a high and dangerous level of
ochratoxin A and total aflatoxins. Our food and fodder are dangerous and our legislation
is way too permissive. We have to align our legislation with the European one to take
care about our grains.
REFERENCES
1. Boariu Carmen, 1988, Cercetări privind modificările fizico – chimice şi biologice
care au loc în timpul păstrării seminţelor destinate consumului., în „Contribuţii ale
cercetării ştiinţifice la dezvoltarea agriculturii din zona centrală a Câmpiei de
Vest”, C.M.D.P.A., 291-318.
2. Braicu Cornelia, Carmen Puia, C. Bele C., E. Bodoki, Carmen Socaciu, 2005,
Optimisation of screening systems to evaluate relevant mycotoxins from cereals
and bread, 4 th <strong>International</strong> Symposium of USAMV Cluj-Napoca, 16:144-149
3. Domsch K.H., W. Gams, 1972, Fungi in agricultural soils, published by Longman.
4. Hulea Ana, 1969, Ghid pentru laboratoarele de micologie şi bacteriologie, Ed.
Agrosilvică, Bucureşti, 304 p.
5. Hulea Ana, Gh. Tasca, C. Beratlief, 1982, Bolile şi dăunătorii produselor agricole
şi hortiviticole după recoltare, Ed. Ceres, Buc., 193 – 210.
6. Mills J.T., 1990, Mycotoxins and toxigenic fungi on cereal grains in western
Canada., Can. J. Physiol. Pharmacol. 1990 Jul; 68 (7):982-6.
7. Raicu Cristina, Doina Baciu, 1978, Patologia semintei, Ed. Ceres, Bucuresti, 207 p.
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