Joint International Conference on Long-term Experiments ...

Class Genotype
Table 1: Regenerating capacity of some wheat breeding lines
48 ours later exposure to high Al 3+ rates
Root elongation (mm)
10 ppp 20 ppm 40 ppm
Al 3+
Al 3+
Al 3+
1 32 B 822 31,0±1,4 15,2±2,4 4,6±2,5
2 14 I 323 11,1±1,1 12,5±5,0 8,7±1,2
3 81 J 475 12,5±2,8 6,0±2,0 0,4±0,3
4 CRISANA 12,0±1,3 4,9±1,0 0,4±0,3
5 138 C 412 17,3±2,9 0 0
6 5 E 122 15,2±6,4 0 0
7 4 K 464 9,2±1,4 0,4±0,2 0
8 88 I 159 5,2±1,4 0 0
9 21 H 454 4,2±1,4 1,4±1,7 0
10 8 J 1010 4,5±1,5 1,5±0,5 0
11 10 K 487 1,4±1,2 0,4±0,4 0
12 29 D 125 1,8±0,9 0 0
13 19 I 197 1,6±0,3 2,9±0,9 0
14 93 J 222 0,3±0,3 0 0
15 10 F 414 0 0 0
16 32 F 132 0 0 0
17 35 F 133 0 0 0
18 76 J 545 0 0 0
19 128 G 128 0 0 0
20 FUNDULEA 29 0 0 0
Estimation
Very tolerant
Tolerant
Susceptible
From a large list of breeding lines created to have a high tolerance to aluminum ions
toxicity, some of them were evidentiated everytime, in any method of testing. Thus,
Oradea 133 line (the futhure variety Crisana) restarted his elongation of roots, even at
40 ppm Al 3+ in solution (table 1).
Another method are based on maximal roots length of plants at 4 ppm Al 3+ , after
10 days of exposure (table 2). The hydroponic solution consist only in distiled water and
aluminium ions, assured from AlCl 3, withouth any other mineral, at 4.5 pH, the
nutritive elements being assured by reserve elements from seed. This method is an
original one, adapted by us for a large series of breeding individuals in segregating
populations.
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