Gilson guide to pipetting - Emidio Albertini

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capillary • Push button • Volume adjustment knob • Ejector of capillary and piston Volumeter display Shaft (also known as capillary holder) Disposable capillary and piston piston AA50002 Serial number MICROMAN p ositive - displacement pipette
6 Selecting the best pipette for your application Chapter 1 Today, there is a pipette for virtually every requirement... aqueous solutions, dense, viscous, or radioactive compounds, DNA sequencing, distributing aliquots, filling microtiter plates, etc. The type of analysis you are performing, the physical properties of the liquid and the volume range of the pipette will determine which pipette and tip you should use. Aqueous samples Viscous samples Radioactive corrosive… samples DNA - RNA biological samples
Page 1 and 2: Gilson guide to pipetting Second Ed
Page 3 and 4: Chapter 1 Selecting the best pipett
Page 5: Air- displacement pipette PIPETMAN
Page 9 and 10: 8 1 .2 The working principle of air
Page 11 and 12: 10 How do air-displacement pipettes
Page 13 and 14: 12 Learning more All air-displaceme
Page 15 and 16: 14 Consider the volume of liquid yo
Page 17 and 18: 16 Selecting the right tip for your
Page 19 and 20: 18 2.2 Choosing the best tip for yo
Page 21 and 22: 20 Correct operation Chapter 3 To o
Page 23 and 24: 22 Fit a disposable pipette tip Pip
Page 25 and 26: 24 3 .3 Adjust the volume setting 1
Page 27 and 28: 26 Air-displacement / Forward mode
Page 29 and 30: 28 Air-displacement / Reverse mode
Page 31 and 32: 30 Positive-displacement In positiv
Page 33 and 34: 32 3 .5 Eject the used tip and stor
Page 35 and 36: 34 Preventing contamination Chapter
Page 37 and 38: 36 4.2 Decontaminating your Pipetma
Page 39 and 40: 38 Caring for your pipette Chapter
Page 41 and 42: 40 Step 2- Assess pipetting functio
Page 43 and 44: 42 Step 4- Disassembly n Disassembl
Page 45 and 46: 44 Control and calibration Chapter
Page 47 and 48: 46 6.2 How to calculate volumetric
Page 49 and 50: 48 Some remarks about balances With
Page 51 and 52: 50 GLOSSARY Accuracy* Air cushion a
Page 53 and 54: 52 APPENDIX APPENDIX I - Example of
Page 55 and 56: 54 APPENDIX APPENDIX III - Evaporat
Page 57 and 58:
56 NOTES
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Gilson guide to pipetting - Emidio Albertini

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