Gilson guide to pipetting - Emidio Albertini
Gilson guide to pipetting - Emidio Albertini
Gilson guide to pipetting - Emidio Albertini
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Chapter 4<br />
Preventing contamination<br />
Types of contamination and how <strong>to</strong> prevent them - - - - - - - - - - - - - - - - - - - - - - - - - - 35<br />
n Personal exposure<br />
n Pipette-<strong>to</strong>-sample<br />
n Sample-<strong>to</strong>-pipette<br />
n Sample-<strong>to</strong>-sample<br />
Decontaminating your Pipetman - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 36<br />
Chapter 5<br />
Caring for your pipette<br />
Quick Diagnosis • The Pipetman Two-minute Inspection- - - - - - - - - - - - - - - - - - - - - - 39<br />
Learning more… How old is my pipette?<br />
Learning more… How can I prevent pis<strong>to</strong>n corrosion?<br />
Learning more… Tips do not fit well. What should I do?<br />
Learning more… What are the main causes of leaks?<br />
Learning more… How do organic solvents cause leaks?<br />
Repair in the lab or return for service? - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 43<br />
Chapter 6<br />
Control and calibration<br />
What are published specifications? - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 45<br />
Learning more… What is accuracy? What is precision?<br />
How <strong>to</strong> calculate volumetric accuracy and precision - - - - - - - - - - - - - - - - - - - - - - - - 46<br />
Using the gravimetric method - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 47<br />
Performance check procedure- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 49<br />
When <strong>to</strong> perform the test- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 49<br />
Glossary - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 50<br />
Appendix<br />
APPENDIX I • Example of a performance check - - - - - - - - - - - - - - - - - - - - - - - - - - - - 52<br />
APPENDIX II • Z fac<strong>to</strong>r - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 53<br />
APPENDIX III • Evaporation loss - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 54
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