Gilson guide to pipetting - Emidio Albertini

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Learning more QWhy do I need a pipette holder? ATo prevent corrosion, contamination and breakage Do not leave your pipette lying on the workbench where it can come into contact with chemicals or fall off and break. Always store your pipette vertically to prevent liquids from running inside the shaft of the pipette. Chapter 3 33
34 Preventing contamination Chapter 4 Avoidance of contamination in the laboratory requires the use of strict precautions. Among these are decontamination of pipettors, wearing gloves and choosing an appropriate pipette tip. The importance of these precautions is evident when we consider the extreme sensitivity of modern techniques such as PCR, which allows detection of a single molecule. We must also bear in mind the dangers of radioactivity and the risk of personal contamination from a pathogenic micro-organism. Personal exposure n Prevention • Wear a lab coat. • Wear gloves. • Wear protective glasses. • Wear a mask. • Wipe work bench before and after with an appropriate cleaner for your application (cell culture, radio-active components, pathogenic samples…). • Work under hood. • Work behind a radioactivity shield. • Avoid touching used tips. • Use unbreakable capillaries and pistons.
Page 1 and 2: Gilson guide to pipetting Second Ed
Page 3 and 4: Chapter 1 Selecting the best pipett
Page 5 and 6: Air- displacement pipette PIPETMAN
Page 7 and 8: 6 Selecting the best pipette for yo
Page 9 and 10: 8 1 .2 The working principle of air
Page 11 and 12: 10 How do air-displacement pipettes
Page 13 and 14: 12 Learning more All air-displaceme
Page 15 and 16: 14 Consider the volume of liquid yo
Page 17 and 18: 16 Selecting the right tip for your
Page 19 and 20: 18 2.2 Choosing the best tip for yo
Page 21 and 22: 20 Correct operation Chapter 3 To o
Page 23 and 24: 22 Fit a disposable pipette tip Pip
Page 25 and 26: 24 3 .3 Adjust the volume setting 1
Page 27 and 28: 26 Air-displacement / Forward mode
Page 29 and 30: 28 Air-displacement / Reverse mode
Page 31 and 32: 30 Positive-displacement In positiv
Page 33: 32 3 .5 Eject the used tip and stor
Page 37 and 38: 36 4.2 Decontaminating your Pipetma
Page 39 and 40: 38 Caring for your pipette Chapter
Page 41 and 42: 40 Step 2- Assess pipetting functio
Page 43 and 44: 42 Step 4- Disassembly n Disassembl
Page 45 and 46: 44 Control and calibration Chapter
Page 47 and 48: 46 6.2 How to calculate volumetric
Page 49 and 50: 48 Some remarks about balances With
Page 51 and 52: 50 GLOSSARY Accuracy* Air cushion a
Page 53 and 54: 52 APPENDIX APPENDIX I - Example of
Page 55 and 56: 54 APPENDIX APPENDIX III - Evaporat
Page 57 and 58: 56 NOTES

Gilson guide to pipetting - Emidio Albertini

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