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Gilson guide to pipetting - Emidio Albertini

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3<br />

Distribution<br />

Press the plunger but<strong>to</strong>n <strong>to</strong> the<br />

first s<strong>to</strong>p. The pis<strong>to</strong>n moves down<br />

and expels the liquid out of the<br />

capillary.<br />

4<br />

Ejection<br />

Press the plunger all the way<br />

down <strong>to</strong> the second and last<br />

s<strong>to</strong>p. Capillary and pis<strong>to</strong>n are<br />

ejected without hand contact.<br />

rest position<br />

first s<strong>to</strong>p<br />

ejection<br />

Important<br />

The pis<strong>to</strong>n and capillary<br />

are the volumetric<br />

components of positive<br />

displacement pipettes.<br />

As both parts are in<br />

contact with liquid, they<br />

must both be replaced<br />

frequently <strong>to</strong> avoid cross-<br />

contamination.<br />

Chapter 3<br />

31

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