Gilson guide to pipetting - Emidio Albertini

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Learning more How to prevent aerosol contamination? It is indispensable to prevent aerosol contamination when you are using PCR and other amplification methods, or when you are pipetting DNA/RNA solutions, infectious materials, radioactive samples, etc. Gilson offers two solutions: Pipetman Microman 1 Use a Pipetman pipette with Diamond presterilized filter tips when you have to face at least two of the following problems at the same time: • working in sterile conditions • pipetting aqueous samples • avoiding cross-contamination. 2 Use a Microman pipette with pre-sterilized capillaries and pistons when you have to face at least two of the following problems at the same time: • working in sterile conditions • pipetting viscous samples • avoiding cross-contamination. For more information, see page 35. Chapter 2 19
20 Correct operation Chapter 3 To optimize your results, the pipette is important but don’t forget to use it properly. 1 First organize your work station for maximum efficiency and minimum fatigue. 2 Make sure the tip is properly mounted and fits well before you set the volume. 3 Adjust the volume. 4 Choose the mode of pipetting (reverse or forward mode) adapted to your sample. 5 Eject the used tip and store the pipette in an upright position to avoid damage and cross-contamination. Take a few minutes to get organized • Adjust your chair or stool so that the work surface is at the right height when you are sitting straight • If possible, always try to work with your hands below shoulder height • Try to evaluate if you can reduce the height of applications such as gel loading • Adjustable tables/workbenches are a good solution Important A good test is to see if you can rest your elbow comfortably on the work surface Recipient at right height Recipient too high Recipient too low
Page 1 and 2: Gilson guide to pipetting Second Ed
Page 3 and 4: Chapter 1 Selecting the best pipett
Page 5 and 6: Air- displacement pipette PIPETMAN
Page 7 and 8: 6 Selecting the best pipette for yo
Page 9 and 10: 8 1 .2 The working principle of air
Page 11 and 12: 10 How do air-displacement pipettes
Page 13 and 14: 12 Learning more All air-displaceme
Page 15 and 16: 14 Consider the volume of liquid yo
Page 17 and 18: 16 Selecting the right tip for your
Page 19: 18 2.2 Choosing the best tip for yo
Page 23 and 24: 22 Fit a disposable pipette tip Pip
Page 25 and 26: 24 3 .3 Adjust the volume setting 1
Page 27 and 28: 26 Air-displacement / Forward mode
Page 29 and 30: 28 Air-displacement / Reverse mode
Page 31 and 32: 30 Positive-displacement In positiv
Page 33 and 34: 32 3 .5 Eject the used tip and stor
Page 35 and 36: 34 Preventing contamination Chapter
Page 37 and 38: 36 4.2 Decontaminating your Pipetma
Page 39 and 40: 38 Caring for your pipette Chapter
Page 41 and 42: 40 Step 2- Assess pipetting functio
Page 43 and 44: 42 Step 4- Disassembly n Disassembl
Page 45 and 46: 44 Control and calibration Chapter
Page 47 and 48: 46 6.2 How to calculate volumetric
Page 49 and 50: 48 Some remarks about balances With
Page 51 and 52: 50 GLOSSARY Accuracy* Air cushion a
Page 53 and 54: 52 APPENDIX APPENDIX I - Example of
Page 55 and 56: 54 APPENDIX APPENDIX III - Evaporat
Page 57 and 58: 56 NOTES

Gilson guide to pipetting - Emidio Albertini

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