Gilson guide to pipetting - Emidio Albertini

2 .1
Evaluating tips
Although they may look alike, all tips are not
the same. The choice of a poor quality tip will
jeopardize your results. Prefer a tip recommended
by the pipette manufacturer and always check the
following points:
Tip Evaluation Sheet
Physical Aspects yes no
Clean and free of dust particles?. . . . . . . . . .
Perfectly formed collar? . . . . . . . . . . . . . . . . .
(for air-tight fit)
Smooth and regular surface? . . . . . . . . . . . .
(minimal retention of liquid)
Fine point finish? . . . . . . . . . . . . . . . . . . . . . .
(prevents formation of droplets)
Translucent . . . . . . . . . . . . . . . . . . . . . . . . . . .
(no contaminating additives or dyes)
Chemical resistance . . . . . . . . . . . . . . . . . . . .
Tip manufacturer’s Guarantee yes no
Brand marked on the tip? . . . . . . . . . . . . . . .
Identification number on the tip? . . . . . . . . .
Batch number on the box? . . . . . . . . . . . . . .
Quality certificate? . . . . . . . . . . . . . . . . . . . . .
Important
Gilson Diamond tips are supplied
with typical values for trace metal release.
These concentrations are lowered
to the analytical noise level after
5 to 10 rinsings with concentrated acid.
Learning more
imperfect
point
aperture
More than meets
the eye
These photos taken under
the microscope show
some of the differences
between good and poor
quality tips.
perfect point
rough
surface
excess
plastic
Chapter 2
17