Gilson guide to pipetting - Emidio Albertini

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Learning more Mistake-free pipetting With Microman pipettes, the piston comes into direct contact (positive-displacement) with the sample. There is no air cushion to expand or contract in response to the density or the temperature of samples. The desired volume is aspirated completely. The piston seal prevents biohazardous, radioactive or corrosive aerosols from entering the instrument. During dispensing, the piston wipes the internal capillary wall, assuring accurate dispensing of almost any viscous sample, from glycerol to glue. To prevent contamination, the capillary and piston may be changed after each sample. They are ejected automatically so… … you do not have to touch them. Chapter 1 13
14 Consider the volume of liquid you want to transfer Pipetman 1 .3 Ultra Pipetman Microman Distriman The eight models of the Gilson Pipetman and Pipetman Ultra are designed to transfer precise volumes from 0.1 µl to 10 ml. Pipetman is also available in fixed volumes from 2 µl to 1 ml. model volume range P2 / U2 0.2 - 2 µl* P10 / U10 1 - 10 µl* P20 / U20 2 - 20 µl P100 / U100 20 - 100 µl P200 / U200 50 - 200 µl* / 20 - 200 µl P1000 / U1000 200 - 1000 µl P5000 / U5000 1 - 5 ml P10ml / U10ml 1 - 10 ml * With a precise pipetting technique P2 and U2 may be used to aspirate volumes down to 0.1 µl, P10 and U10 at 0.5 µl, and P200 at 30 µl. The six models of the Gilson Microman speciality pipettes are designed for the precise transfer of problem liquids in volumes from 1 µl to 1000 µl. model volume range M10 1 - 10 µl M25 3 - 25 µl M50 20 - 50 µl M100 10 - 100 µl M250 50 - 250 µl M1000 100 - 1000 µl The Distriman repetitive pipette is available with three different syringes (micro, mini and maxi), so you can distribute aliquots in any volume (even fractional) from 1 µl to 1.25 ml. syringe volume range (total syringe volume) of aliquots Micro (125 µl) 1 to 12.5 µl Mini (1250 µl) 10 to 125 µl Maxi (12.5 ml) 100 µl to 1.25 ml Important The model and the useful volume range on Pipetman, Pipetman Ultra and Microman pipettes are shown on the top of the push-button Important The total volume that can be aspirated is shown on the side of each Distriman syringe
Page 1 and 2: Gilson guide to pipetting Second Ed
Page 3 and 4: Chapter 1 Selecting the best pipett
Page 5 and 6: Air- displacement pipette PIPETMAN
Page 7 and 8: 6 Selecting the best pipette for yo
Page 9 and 10: 8 1 .2 The working principle of air
Page 11 and 12: 10 How do air-displacement pipettes
Page 13: 12 Learning more All air-displaceme
Page 17 and 18: 16 Selecting the right tip for your
Page 19 and 20: 18 2.2 Choosing the best tip for yo
Page 21 and 22: 20 Correct operation Chapter 3 To o
Page 23 and 24: 22 Fit a disposable pipette tip Pip
Page 25 and 26: 24 3 .3 Adjust the volume setting 1
Page 27 and 28: 26 Air-displacement / Forward mode
Page 29 and 30: 28 Air-displacement / Reverse mode
Page 31 and 32: 30 Positive-displacement In positiv
Page 33 and 34: 32 3 .5 Eject the used tip and stor
Page 35 and 36: 34 Preventing contamination Chapter
Page 37 and 38: 36 4.2 Decontaminating your Pipetma
Page 39 and 40: 38 Caring for your pipette Chapter
Page 41 and 42: 40 Step 2- Assess pipetting functio
Page 43 and 44: 42 Step 4- Disassembly n Disassembl
Page 45 and 46: 44 Control and calibration Chapter
Page 47 and 48: 46 6.2 How to calculate volumetric
Page 49 and 50: 48 Some remarks about balances With
Page 51 and 52: 50 GLOSSARY Accuracy* Air cushion a
Page 53 and 54: 52 APPENDIX APPENDIX I - Example of
Page 55 and 56: 54 APPENDIX APPENDIX III - Evaporat
Page 57 and 58: 56 NOTES

Gilson guide to pipetting - Emidio Albertini

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