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Seedling Growth of Gmelina Arborea (Roxb) as Influenced by Crude Oil in Soil 406 [20] .Keay, R.W.J. 1989. Trees of Nigeria: a revised version of Nigerian trees (volumes 1 and 2). Clarenden Press, Oxford. [21] Statistical Analytical System (SAS) 1996 user’s guide. Statistics version 5, SAS Institute Inc. Raleigh, N.Y., USA. 1996. [22] Schwendinger, R.B. (1968). Reclamation of soil contaminated with oil. Journal Institute Petroleum 54 (535): 182-197. [23] Udo, E. J. and Oputa, C. O. 1984. Some studies on the effect of crude oil pollution of soil on plant growth. Journal of Biology and Applied Chemistry 29: 3 – 14.
European Journal of Scientific Research ISSN 1450-216X Vol.14 No.3 (2006), pp. 407-416 © EuroJournals Publishing, Inc. 2006 http://www.eurojournals.com/ejsr.htm A Comparative Analysis of Gibberellic Acid Content with Respect to Tuber Induction in Potato Plants Grown Under Differential Photoperiod and Temperature Ahmed Malkawi Department of Chemistry and Physics Northwest Missouri State University Maryville, MO 64468, USA E-mail: amalkaw@nwmissouri.edu Abstract A time-course experiment was performed to determine the effect of photoperiod and temperature on endogenous gibberellic acid (GA3) levels in vegetative tissues of potato (Solanum tuberosum cultivar “Russet Burbank”) in search of an unequivocal role for GA3 in tuber induction. Plants were initially grown in tuber non-inducing climatic conditions (30ºC day and 26ºC night with an 18-hour photoperiod) until they reached a height of 55 to 60 cm after which half of the plants were transferred to inducing climatic conditions (24ºC day and 12ºC night with a 10-hour photoperiod). Harvest of each plant type began 48 hours later and over a period of 10 days with 2-day intervals. Plants were divided into aerial and underground tissues, freeze-dried, ground, and then subjected to a purification procedure involving solvent extraction and high performance liquid chromatography. The purified GA3 extract was then quantified by using combined gas chromatography-mass spectrometry. Levels of GA3 declined progressively during the ten-day tuber induction period while it slightly increased under non-inducing conditions progressively. Our study establishes a strong correlation between decreased GA3 level and tuber induction. Keywords: chromatography, gibberellic acid, plant hormone, potato, Solanum tuberosum Introduction Potato tuberization is the physiological process whereby a stem section of a plant undergoes morphological changes to become a special storage organ for reserve food such as carbohydrates and proteins (Melis and van Staden 1984; Ewing and Struik 1992). Under optimum environmental conditions for potato plants to switch from vegetative development to reproductive growth, a signal formed in the leaves is transmitted through the phloem to the underground stems, called stolons (Jackson 1999; Suarez-Lopez 2005 Rodriguez-Falcon et al. 2006). The synthesis of such signal and its translocation is referred to as the induction of tuberization and signal is known as the “tuberization stimulus”. The leaf-derived signal can be transmitted across a graft union, as demonstrated in experiments involving ordinary plants (Gregory 1956; Chapman 1958; Kumar and Wareing 1973) as well as transgenic plants (Jackson et al. 1998). The induction of tubers is considered a key developmental stage in the potato crop’s life cycle, with “profound implications for subsequent growth and development” (O’Brian et al. 1998).
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A Comparative Analysis of Gibberell
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Nutritive Evaluation of Some Trees
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Determination of Sample Size 320 mi
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Page 86 and 87: 390 Amani Michel Kouassi, Koffi Fer
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