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IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />

Fremantle, Western Australia<br />

27 November – 1 December 2011<br />

Session Details: Wednesday 30 November 2011<br />

Concurrent Session 15 – Quantification of N-<strong>Fixation</strong><br />

1600 – 1740<br />

Authors: Salmina N. Mokgehle 1 , Cherian Mathews 2 and Felix D. Dakora 3<br />

1 Department of Crop Sciences, Tshwane University of Technology, Private Bag X680,<br />

Pretoria 0001, South Africa.<br />

2 Department of Agriculture, Rural Development and Land Administration, Private Bag<br />

X11318, Nelspruit, 1200<br />

3 Department of Chemistry, Tshwane University of Technology, Private Bag X680,<br />

Pretoria 0001, South Africa.<br />

Presentation Title: Selecting field plants for drought tolerance and N2 fixation in 25 groundnut (Arachis<br />

hypogaea<br />

13C and 15N natural abundance<br />

Presentation Time: 1700 – 1720<br />

Groundnut is a major commercial grain legume in Africa, often cultivated for its seed oil and high protein content.<br />

Although ICRISAT has historically selected groundnut genotypes for grain yield and disease resistance in Africa,<br />

little is known about their N contribution and drought tolerance under local conditions. In this study, field trials<br />

were conducted at three sites (namely, Nelspruit, Mzinti and Kliplaatdrift) within Mpumalanga Province of South<br />

Africa in 2009. The data revealed significant differences in plant biomass, N concentration, and N content at all<br />

three sites. The δ 15 N values of groundnut genotypes ranged from -0.08‰ to +1.06‰ at Nelspruit, +0.41‰ to<br />

+0.95‰ at Mzinti, and +0.73‰ to +1.96‰ at Kliplaatdrift. About 24 out of the 25 groundnut genotypes obtained<br />

over 50% of their N nutrition from symbiotic fixation at Mzinti, while at Kliplaatdrift only five genotypes derived<br />

50% or more of their N nutrition from symbiotic fixation. The amounts of N-fixed at Nelspruit ranged from 76<br />

kg/ha for ICGV99033 and PC327K31 to 188 kg/ha for ICGV00362. At Mzinti, 10 genotypes contributed over 100<br />

kg N /ha, while at Kliplaatdrift only 5 genotypes fixed more than 100 kg/ha. Data on ∂ 13 C values also revealed<br />

marked difference in drought tolerance. Genotypes ICGV03157 and ICGV00369, for example, showed much<br />

lower 13 C discrimination at all three sites, indicating greater water-use efficiency compared to the remaining<br />

genotypes.<br />

95<br />

2011

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