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IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />

Fremantle, Western Australia<br />

27 November – 1 December 2011<br />

Session Details:<br />

Wednesday 30 November 2011<br />

Concurrent Session 14 – Cyanobacteria & other organisms<br />

1600 - 1740<br />

Authors: Junko Tazawa 1 , Yoshinari Ohwaki 1 , Tadashi Yokoyama 2 , Yoshiaki Shizukawa 3 , Ui<br />

Ono 3 & Masami Yoshikawa 3<br />

1 NARO Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan<br />

2 Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan<br />

3 Biotechnology Research Department, Kyoto Prefectural Agriculture, Forestry and<br />

Fisheries Technology Center, Seika, Kyoto 619-0244, Japan<br />

Presentation Title: Endophytic occupation of soybean nodules by Rhizobium and other bacterial species<br />

under continuous cultivating conditions<br />

Presentation Time: 1720 – 1740<br />

Rhizobial bacteria can form nodules and establish symbiosis on the roots of their leguminous host plants.<br />

Recently, several reports have stated that some non-symbiotic bacteria occupy legume nodules endophyticly,<br />

although the function of these bacteria in the nodules has not been understood. We have isolated non-symbiotic<br />

bacteria from root nodules of soybean (cv: Hatayutaka) grown in a continuously cultivated field. Fast-growing<br />

bacterial strains were isolated from surface-sterilized nodules on YMA medium as non-symbiotic endophytic<br />

bacteria. Phylogenetic analysis based on the partial sequence of 16S rDNA gene demonstrated that most of<br />

these isolates were close to the genera Rhizobium/Agrobacterium, Bacillus, Paenibacillus, Enterobacter, and<br />

Pseudomonas. The percentage of nodules that coexisted with symbiotic Bradyrhizobium strains and nonsymbiotic<br />

bacteria was very low (

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