IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />
Fremantle, Western Australia<br />
27 November – 1 December 2011<br />
Session Details:<br />
Wednesday 30 November 2011<br />
Concurrent Session 14 – Cyanobacteria & other organisms<br />
1600 - 1740<br />
Authors: Junko Tazawa 1 , Yoshinari Ohwaki 1 , Tadashi Yokoyama 2 , Yoshiaki Shizukawa 3 , Ui<br />
Ono 3 & Masami Yoshikawa 3<br />
1 NARO Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan<br />
2 Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan<br />
3 Biotechnology Research Department, Kyoto Prefectural Agriculture, Forestry and<br />
Fisheries Technology Center, Seika, Kyoto 619-0244, Japan<br />
Presentation Title: Endophytic occupation of soybean nodules by Rhizobium and other bacterial species<br />
under continuous cultivating conditions<br />
Presentation Time: 1720 – 1740<br />
Rhizobial bacteria can form nodules and establish symbiosis on the roots of their leguminous host plants.<br />
Recently, several reports have stated that some non-symbiotic bacteria occupy legume nodules endophyticly,<br />
although the function of these bacteria in the nodules has not been understood. We have isolated non-symbiotic<br />
bacteria from root nodules of soybean (cv: Hatayutaka) grown in a continuously cultivated field. Fast-growing<br />
bacterial strains were isolated from surface-sterilized nodules on YMA medium as non-symbiotic endophytic<br />
bacteria. Phylogenetic analysis based on the partial sequence of 16S rDNA gene demonstrated that most of<br />
these isolates were close to the genera Rhizobium/Agrobacterium, Bacillus, Paenibacillus, Enterobacter, and<br />
Pseudomonas. The percentage of nodules that coexisted with symbiotic Bradyrhizobium strains and nonsymbiotic<br />
bacteria was very low (