IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ... IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Session Details: Wednesday 30 November 2011 Concurrent Session 12 – PGPR & Plant Production 1100 – 1230 Authors: Khanok-on Amprayn and Ivan Kennedy SUNFix Centre for Nitrogen Fixation University of Sydney, NSW 2006, Australia Presentation Title: Proteomics of rice-PGP microorganisms' interactions Presentation Time: 1120 - 1140 A plant growth-promoting (PGP) bacterium Pseudomonas fluorescens 1N (Pf1N) and a soil yeast Candida tropicalis HY (CtHY) have been used as two of the components of a commercial biofertiliser named BioGro to enhance growth and yield of rice allowing reduce chemical applications in Vietnam for many years. The present study verified the colonisation on rice roots and the effects of the strains on rice root mechanisms as observable morphological changes were correlated with results by proteomics. In addition, bacterial proteins mediating the interaction between rice root and Pf1N were investigated. To explain the strains influence on rice root growth, gel-based comparative proteomics and mass spectrometry were employed. A comparison of 2-DE protein profiles between untreated control and PGP microbes treated roots revealed that the inoculation of the Pf1N contributed 11 differentially expressed proteins in rice roots, while 20 and 18 root proteins were differentially expressed in CtHY and co-inoculation of Pf1N and CtHY treatment. Of these changed proteins, the identity of 20 proteins was determined by MALDI TOF MS. The protein identification revealed that responsive plant proteins belonged to eight major metabolic processes including catabolism and respiration (energy production), transcription, protein synthesis, protein destination and storage, secondary metabolism, membrane transporters, disease-defense, and unknown. Conversely, the bacterial proteome alteration in presence of root exudates was also profiled using 2-DE and MS to examine of bacterial adaptation in association with rice. The bacterial protein expressions revealed several mechanisms involved in protein transcription and synthesis, primary metabolism, cell envelope development, siderophore production, nutrient transportation, general stress response, and unknown. The patterns of protein expression of Pf1N such as elevated iron uptake mechanisms, repressed essential growth-related proteins and ABC transporter suggested that the bacterium was adapted to form biofilm. 76 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Session Details: Wednesday 30 November 2011 Concurrent Session 12 – PGPR & Plant Production 1100 – 1230 Authors: Iris Marcano 1 , César A. Díaz-Alcántara 1 , Beatriz Urbano 2 , Encarna Velázquez 3 , Fernando González-Andrés 4 . 1 Facultad de CC. Agronómicas y Veterinarias. Univ. Autónoma de Santo Domingo. Dominican Republic. 2 Departamento de Ingeniería Agrícola y Forestal. Universidad de Valladolid. Spain. 3 Departamento de Microbiología y Genética. Universidad de Salamanca. Spain. 4 Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad. Universidad de León. Spain. Presentation Title: In vitro plant growth promotion properties of endophitic bacteria isolated from banana (Musa sp.) roots in Dominican Republic Presentation Time: 1140 - 1200 Organic agriculture, using rizospheric or endophytic bacteria which promote plants growth (PGPR) is one of the chief strategies to reduce the use of Chemicals in agriculture. The present study had the objective of evaluates endophytic rhizobacteria isolated from banana roots (Musa sp) and other crops, from four provinces in Dominican Republic (Mao, Azua, Montecristi and Dajabón), in order to make a preselection of bacterial strains to elaborate biofertilisers for banano organic crops in Dominican Republic. The total number of isolates was 160. The RAPD pattern obtained after amplification of the DNA with the primer M13 was used to identify possible clones, and the collection was reduced to 144 different strains. The 144 strains were evaluated in vitro by their efficiency producing the hormone IAA, the evidence of siderophores production and the ability of solubilise insoluble mineral phosphate. Sixteen per cent of the 144 strains produced significant rates of IAA, 22% produced siderophores and 11% solubilized P. Only 14 strains solubilised inorganic P and produced siderophores at the same time, and none of them were among the best producers of IAA, indicating that it is not common to find strains with several in vitro plant growth promotion properties simultaneously. The strains with highest plant growth promotion properties in vitro have been identified by 16S rRNA sequencing and comparison with international databases, to ensure that they belong to non-pathogenic taxa, following the phase of in vivo selection. By the moment, 6 of the 14 strains that solubilize inorganic P and produce siderophores, were identified, and 4 belonged to the species Pseudomonas taiwanensis, one to P. mosselii and one to Enterobacter absuriae, all of them common endophytic bacterial taxa (Dobbelaere et al., 2003). Dobbelaere, S. Vanderleyden, J., Okon, Y. 2003. Plant Growth-Promotign Effects of Diazotrophs in the Rhizosphere. Critical Reveiws in Plant Sciences, 22 (2): 107-149. 77 2011
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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />
Fremantle, Western Australia<br />
27 November – 1 December 2011<br />
Session Details: Wednesday 30 November 2011<br />
Concurrent Session 12 – PGPR & Plant Production<br />
1100 – 1230<br />
Authors: Iris Marcano 1 , César A. Díaz-Alcántara 1 , Beatriz Urbano 2 , Encarna Velázquez 3 , Fernando<br />
González-Andrés 4 .<br />
1 Facultad de CC. Agronómicas y Veterinarias. Univ. Autónoma de Santo Domingo.<br />
Dominican Republic.<br />
2 Departamento de Ingeniería Agrícola y Forestal. Universidad de Valladolid. Spain.<br />
3 Departamento de Microbiología y Genética. Universidad de Salamanca. Spain.<br />
4 Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad. Universidad de León.<br />
Spain.<br />
Presentation Title: In vitro plant growth promotion properties of endophitic bacteria isolated from banana<br />
(Musa sp.) roots in Dominican Republic<br />
Presentation Time: 1140 - 1200<br />
Organic agriculture, using rizospheric or endophytic bacteria which promote plants growth (PGPR) is one of the<br />
chief strategies to reduce the use of Chemicals in agriculture. The present study had the objective of evaluates<br />
endophytic rhizobacteria isolated from banana roots (Musa sp) and other crops, from four provinces in<br />
Dominican Republic (Mao, Azua, Montecristi and Dajabón), in order to make a preselection of bacterial strains to<br />
elaborate biofertilisers for banano organic crops in Dominican Republic. The total number of isolates was 160.<br />
The RAPD pattern obtained after amplification of the DNA with the primer M13 was used to identify possible<br />
clones, and the collection was reduced to 144 different strains. The 144 strains were evaluated in vitro by their<br />
efficiency producing the hormone IAA, the evidence of siderophores production and the ability of solubilise<br />
insoluble mineral phosphate. Sixteen per cent of the 144 strains produced significant rates of IAA, 22% produced<br />
siderophores and 11% solubilized P. Only 14 strains solubilised inorganic P and produced siderophores at the<br />
same time, and none of them were among the best producers of IAA, indicating that it is not common to find<br />
strains with several in vitro plant growth promotion properties simultaneously. The strains with highest plant<br />
growth promotion properties in vitro have been identified by 16S rRNA sequencing and comparison with<br />
international databases, to ensure that they belong to non-pathogenic taxa, following the phase of in vivo<br />
selection. By the moment, 6 of the 14 strains that solubilize inorganic P and produce siderophores, were<br />
identified, and 4 belonged to the species Pseudomonas taiwanensis, one to P. mosselii and one to Enterobacter<br />
absuriae, all of them common endophytic bacterial taxa (Dobbelaere et al., 2003).<br />
Dobbelaere, S. Vanderleyden, J., Okon, Y. 2003. Plant Growth-Promotign Effects of Diazotrophs in the<br />
Rhizosphere. Critical Reveiws in Plant Sciences, 22 (2): 107-149.<br />
77<br />
2011
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