IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ... IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

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KeYNoTe SPeAKeRS Allan Downie After completing a PhD, post-doc and a fellowship in microbial bioenergetics, I moved from Canberra to the John Innes (Norwich) in 1981 and started working on Rhizobium-legume interactions. My work on identifying various nodulation genes included the characterisation of a secreted nodulation protein that forms cation-selective ion channels. This led me to analyse Ca++ changes in legume root hairs in response to Nod factors in WT and nodulation-defective mutants, leading to a proposed Nod-factor activated signalling pathway. More recently I have been trying to understand how Nod-factor-induced Ca++ signalling might be coupled to initiation of rhizobial infection of root hairs. Ken Giller Ken Giller is Professor Plant Production Systems at Wageningen University. He leads a group of scientists with profound experience in systems analysis and simulation modelling scenarios of change. Ken’s research has focused on nitrogen fixation in tropical legumes and he is author of the standard text “Nitrogen Fixation in Tropical Cropping Systems” published in second edition in 2001. He has 5 books, >200 papers in peer-reviewed, international journals (H factor=37) and >100 book chapters. He leads a US$19.2M project “N2Africa – Putting nitrogen fixation to work for smallholder farmers in Africa (www.N2Africa.org)” across 8 countries in Africa funded by the Bill & Melinda Gates Foundation. Giles Oldroyd Western agricultural systems are reliant on the application of inorganic nitrogen fertilisers that greatly enhance yield. However, production and application of nitrogen fertilisers account for a significant proportion of fossil fuel usage in food production and the major global source of nitrous oxide emissions, a very potent greenhouse gas. Prof Giles Oldroyd studies the mechanisms by which some species of plants are capable of forming beneficial interactions with nitrogen fixing bacteria, that provide a natural source of nitrogen for plant growth. A long term aim of this research is to reduce agricultural reliance on nitrogen fertilisers. Giles completed his PhD in 1998 at the University of California, Berkeley, studying plant/pathogen interactions and then moved to Stanford University to complete a postdoc on legume/ rhizobial interactions in the laboratory of Prof. Sharon Long. He has been an independent researcher at the John Innes Centre since 2002. From 2002 to 2007 he held a BBSRC David Philips Fellowship and since 2007 has been a tenured member of the JIC faculty. In 2008 he was made the head of the Institute Strategic Programme Grant “Plant sensing and responding to the Environment” and between 2010-2011 he was the Deputy Director of the John Innes Centre. He has been recognised by a number of awards for his research: EMBO young investigator; European Research Council young investigator; Society of Experimental Biology Presidents medal; Royal Society Wolfson Research Merit award and the BBSRC David Phillips Fellowship. Phil Poole Phil Poole is originally from Perth but slipped up while on the typical Aussie European tour of Europe by staying to do post doctoral work in the biochemistry department at Oxford. From there he moved to Reading and then to the John Innes Centre in 2007. Phil works on the physiology and genetics of root nodule and rhizosphere/soil bacteria. He is particularly interested in: 1) The regulation of nutrient exchange between Rhizobium and their host legumes 2) Development and differentiation of the legume bacteroids 3) The molecular basis of competitive success of bacteria in the plant rhizosphere 6

Cottesloe Beach © Tourism Western Australia Luis M. Rubio Luis M. Rubio is Assistant Professor at the Center for Plant Genomics and Biotechnology of the Technical University of Madrid (Spain). He obtained BS and PhD in Biology from the University of Seville. He worked as posdoctoral researcher at the University of Wisconsin-Madison and as Associate Scientist at the University of California-Berkeley. His main research interest is the biochemistry and biosynthesis of nitrogenase, with a focus on the study of iron-molybdenum cofactor biosynthesis. His ongoing research also includes improvement of biological hydrogen production by altered forms of nitrogenase. Janet Sprent Janet Sprent is Emeritus Professor of Plant Biology in the University of Dundee and Honorary Research Fellow at the James Hutton Institute, Dundee. She has spent decades working on nitrogen cycling and fixation, especially by legumes, working in all continents, including Antarctica. Current collaborations are in Europe, Africa, India and Australia and focus on nodulation processes in understudied legumes. Her talk will have a global coverage of the great variety of legumes, their nodule structure and nodulating bacteria and will speculate on how legumes may have evolved over time to fill particular geographic niches Jens Stougaard Jens Stougaard´s primary research activity is on genes regulating development of nitrogen fixing root nodules and mycorrhiza formation in legumes. Currently the mechanisms of Nod-factor perception, the function of receptors involved and the downstream signal transduction cascades are in focus. The plant model system used for this research is Lotus japonicus that is also used for investigating the long range signalling integrating root nodule development into the general developmental program of the plant. Genetics, genomics and biochemical methods are used to identify and characterise components of regulatory circuits. In order to improve the genetic analysis and to establish a system for reverse genetics, a large-scale insertion population based on the germ-line specific activity of the LORE1 retroelement is being established and made available to the community. Michael Udvardi Michael Udvardi: I earned my PhD at The Australian National University where I worked on the biochemistry of transporters of the symbiosome membrane that mediate traffic of nutrients between plant cells and rhizobia in nitrogen-fixing legume nodules. During my postdoc years, I used molecular biology approaches to isolate and characterize plant genes involved in nodule metabolism. Over the years, my research interests have broadened, although work on symbiotic nitrogen fixation remains a core activity of my lab. I am now a Professor at the Samuel Roberts Noble Foundation where we are developing and applying tools for functional genomics in Medicago truncatula. Using forward and reverse-genetic approaches, we are currently identifying new genes required for nodule development, differentiation, and symbiotic nitrogen fixation. 7 Amberley Estate © Tourism Western Australia

KeYNoTe SPeAKeRS<br />

Allan Downie<br />

After completing a PhD, post-doc and a fellowship in microbial bioenergetics, I moved from Canberra<br />

to the John Innes (Norwich) in 1981 and started working on Rhizobium-legume interactions. My work<br />

on identifying various nodulation genes included the characterisation of a secreted nodulation protein<br />

that forms cation-selective ion channels. This led me to analyse Ca++ changes in legume root hairs in<br />

response to Nod factors in WT and nodulation-defective mutants, leading to a proposed Nod-factor<br />

activated signalling pathway. More recently I have been trying to understand how Nod-factor-induced<br />

Ca++ signalling might be coupled to initiation of rhizobial infection of root hairs.<br />

Ken Giller<br />

Ken Giller is Professor Plant Production Systems at Wageningen University. He leads a group of scientists<br />

with profound experience in systems analysis and simulation modelling scenarios of change. Ken’s<br />

research has focused on nitrogen fixation in tropical legumes and he is author of the standard text<br />

“<strong>Nitrogen</strong> <strong>Fixation</strong> in Tropical Cropping Systems” published in second edition in 2001. He has 5 books,<br />

>200 papers in peer-reviewed, international journals (H factor=37) and >100 book chapters. He leads<br />

a US$19.2M project “N2Africa – Putting nitrogen fixation to work for smallholder farmers in Africa<br />

(www.N2Africa.org)” across 8 countries in Africa funded by the Bill & Melinda Gates Foundation.<br />

Giles Oldroyd<br />

Western agricultural systems are reliant on the application of inorganic nitrogen fertilisers that greatly<br />

enhance yield. However, production and application of nitrogen fertilisers account for a significant<br />

proportion of fossil fuel usage in food production and the major global source of nitrous oxide emissions,<br />

a very potent greenhouse gas. Prof Giles Oldroyd studies the mechanisms by which some species of<br />

plants are capable of forming beneficial interactions with nitrogen fixing bacteria, that provide a natural<br />

source of nitrogen for plant growth. A long term aim of this research is to reduce agricultural reliance on<br />

nitrogen fertilisers. Giles completed his PhD in 1998 at the University of California, Berkeley, studying<br />

plant/pathogen interactions and then moved to Stanford University to complete a postdoc on legume/<br />

rhizobial interactions in the laboratory of Prof. Sharon Long. He has been an independent researcher<br />

at the John Innes Centre since 2002. From 2002 to 2007 he held a BBSRC David Philips Fellowship and<br />

since 2007 has been a tenured member of the JIC faculty. In 2008 he was made the head of the Institute<br />

Strategic Programme Grant “Plant sensing and responding to the Environment” and between 2010-2011<br />

he was the Deputy Director of the John Innes Centre. He has been recognised by a number of awards<br />

for his research: EMBO young investigator; European Research Council young investigator; Society of<br />

Experimental Biology Presidents medal; Royal Society Wolfson Research Merit award and the BBSRC<br />

David Phillips Fellowship.<br />

Phil Poole<br />

Phil Poole is originally from Perth but slipped up while on the typical Aussie European tour of Europe<br />

by staying to do post doctoral work in the biochemistry department at Oxford. From there he moved<br />

to Reading and then to the John Innes Centre in 2007. Phil works on the physiology and genetics of root<br />

nodule and rhizosphere/soil bacteria. He is particularly interested in:<br />

1) The regulation of nutrient exchange between Rhizobium and their host legumes<br />

2) Development and differentiation of the legume bacteroids<br />

3) The molecular basis of competitive success of bacteria in the plant rhizosphere<br />

6

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