IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ... IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Selection of plant growth promoting rhizobacteria (PGPR) to enhance nodulation of grain legumes by rhizobia Author Liza Parkinson 1 , Graham O‟Hara 1 , Ron Yates 1, 2 , Paul Harvey 3 and Lambert Brau 1 Poster Board Number 51 1 Murdoch University, Centre for Rhizobium Studies, Murdoch, Western Australia, 6150 2 Department of Agriculture and Food Western Australia, South Perth, Western Australia, 6151 3 CSIRO, Urrbrae, South Australia, 5064 Plant growth-promoting rhizobacteria (PGPRs) are soil bacteria associated with plant roots that have been shown to stimulate plant growth and crop yield. The mechanisms by which PGPRs increase plant growth include the production of plant hormones, suppression of disease–causing microbes as well as improved plant nutrient availability and assimilation. It has also been shown that the formation of nodules on legumes induced by rhizobia can be enhanced by co-inoculation with PGPRs. To examine the benefits of co-inoculating rhizobia with PGPRs, six newly identified PGPRs will be screened in glasshouse trials on Lupins (Lupinus angustifolius), Peas (Pisum sativum), Lentils (Lens culinaris), Beans (Phaseolus vulgaris), Chickpeas (Cicer arietinum) and Soybeans (Glycine max) in addition to the current commercial rhizobia. PGPRs used in this study include five Pseudomonas species, which have previously been shown to enhance nodulation in legumes and one gram-positive strain isolated from a Lupinus nodule. To evaluate the efficacy of the PGPRs on enhancing the legume-rhizobia symbiosis, the following will be measured: nodule numbers, nodule biomass, shoot and root biomass and shoot nitrogen content. In addition, nodule occupancy will be analyzed using PCR fingerprinting techniques to establish whether PGPRs are able to colocalize with rhizobia in the nodules. Promising isolates from these trails will be selected to elucidate the mechanism by which they enhance nodulation and/or nitrogen fixation. This will include analysis of expression of putative PGP genes, targeted gene knock-outs, and population genetic studies on the microbial community to determine the impact of inoculants on rhizobium populations in the rhizosphere. 166 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Abdou, MM 109 Cao, Q 31 do Nascimento, EC 128 Hartley, E 82, 133 Keakile, KK 130 Abe, M 131, 137,156 Capela, D 37 Dolgikh, EA, 40 Hartley, J 133 Kelly, S 51 Adnane, B 111 Carlson, R 51 Dong, W 26 Hartmann, A 66 Kenicer, G 62 Agarie, S 131 Carlsson , G 101 Downie JA 16 Harvey, P 166 Kennedy, I 76, 78 Aisthorpe, D 47 Carmody, B 45, 152 Drevon, JJ 99, 109, 111 Hashiguchi, M 131 Khojiev, Z 120 Akashi, R 131 Castelli, J 42 Drew, EA 44, 52 Hayashi, M 125, 158 Kieu, LN 103 Alatalo, E 127 Casteriano, A 81, 162 East, A 36 Hayashi, S 57 Kim, J 16 Alexandre, A 104 Catrice, O 37 Echavarri-Erasun, C 17 He, J 50 Kimanthi, M 86 Alves, BJR 97, 128 Chaïbi, S 118 Eda, S 160 He, X 93 Kobryn, H 24 Amaddin, PAM 90 Chansa-Ngavej, K 46 Edwards, A 16 Heckmann, AL 16 Koch, M 41 Amenc, L 109 Charman, N 48, 54, 79 Enoki, A 150 Hennecke, H 41 Kouchi, H 157 Ampomah, OY 62 Chen W-M 67 Erbacher, A 47 Herridge, D 96, 101, 103 Kryvoruchko, I 50 Amprayn, K 76 Chen, C 42, 147 Espinoza, S 94, 121 Herrmann, L 84, 86, 134, Kucho, K 137, 156 Andersen, SU 151 Chen, R 50 Faghire, M 99 Higashi, S 137,156 Kyrpidis, N 34 Andrianananjara, A 109 Chen, T 119 Faraut, T 37 Hiratsuka, Y 131 Lake, L 52 Angus, JF 100 Chen, WF 63 Farquharson, R 48 Hirsch, AM 131 Lazali, M 99 Araragi, M 156 Chen, WX 63 Faye, A 84 Högbom, M 27 Lee, K-T 161 Ardley, JK 69 Chen, X 81 Federova, E 31, 39, 65, 74 Hood, G 36 Lei, L 43 Arima, S 131 Cheng, G 43 Ferguson, B 57 Hori, Y 137 Leppyanen, IV, 40 Arpiwi, NL 107 Cheng, Z 50 Ferreira, L 155 Howieson, JG 15, 24, 49, Lesueur, D 84, 86, 134, 135 167 135 56, 68, 69, 83, 165 Asami, T 156 Chiasson, D 39, 122 Fischer, D 66 Hubber, A 51 Li, D 57 Aserse, AA 145 Chien, H-L 161 Fischer, H-M, 41 Humphries, A 79 Li, M 63 Assefa, F 145 Chimphango, SBM 67 Forster, B 89 Hurek, T 81 Li, QQ 63 Atieno, M 84, 134 Chu, X 163 Fox, S 45, 75 Huss-Danell, K 62, 92 Li, Y 43, 164 Attar, H 99 Clarke, VC 42, 129 Franchini, JC 97 Huwiler, S 41 Liao, S 72 Bakkou, N 38 Cong, PT 103 Gamas, P 37 Huyghe, A 38 Limpens, E 65 Baldani, JI 66 Conway, M 47 García-Benavides, P 154 Hynes, MF 35 Lin, M-H 57 Baldock, J 48 Corbett, MK 106 García-Fraile, P 155 Iannetta, PPM 62 Lin, Y-H 57 Ballard, N 22 Craig, A 54 Garg, N 108 Ikenishi, F 160 Lindström, K,21, 127, 145, 146 Ballard, RA 44, 48, 52, 54, 79 Cramer, SP 26 Gavrin, A 74 Imaizumi-Anraku, H 125 Liu, C-T 161 Balone, T 130 Critchley, C 93 Ge, Y-Y 72 Imin, N 58 Liu, L 164 Banabas, M 96 Cuddy, W 89 Geddes, BA 35 Inaba, S 102, 160 Liu, TY 63 Barbour, EL 107, 148 Dai, X 50 Gehlot, HS 60 Inada, S 131 Loi, A 83 Bargaz, A 99 Dakora, FD 25, 55, 95, 110, Gehringer, MM 89 Ismail, MR 88 Lopatin, SA 40 116, 140, 141, 142, 143, 144 Barrero, R 56 Danza, F 41 Gelfand, M 32 Itakura, M 102, 160 Loughlin, P 39, 42, 122, 129, 147 Beatty, P 98 Dapper, CH 26 Gemell, G 82, 133 Ivanov, S 65, 74 Ludwig, M 42 Belane, A 55, 144 Day, DA 42, 122, 129, 167 Gemmer, S 81 Jalovaja, J 32 Majengo, C 86 Bellgard, M 56 de Araújo, JLS 128 George, SJ 26 James, EK 60, 62, 67 Makhubedu, I 141 Benedito, VA 50, 59 De Carvalho-Niebel, F 37 Gerding, M 68 Jantalia, CP 97, 128 Malik, R 23 Benito, EP 154 de Mita, S 65 Ghazali,, A 136 Jardinaud, F 37 Małolepszy, A 151 Berthold, CL 27 Deaker, R 82, 89, 103, 162 Ghoulam, C 99, 111 Jikumaru, Y 131 Mantaj, M 70 Bisseling, T 31, 39, 65, 74 Debéllé, F 37 Giller, K,19 Jiménez-Vicente, E 17, 29, Mapope, N 142 Bledsoe, C 93 Del Pozo, A 94, 121 Gonzales-Andres, F 77, 153 Jin, L 119 Marcano, I 77 Boddey, RM 97, 128 Delmotte, N 41 González-Buitrago, JM 155 Jing, Y 124 Marques, AT 132 Bonython, A 54 Demina, IV 159 Good, AO 98 Kahn, ML 28 Martínez-Hidalgo, P 154, 155 Boonkerd, N 123, 138, 140 Denton, MD 52, 79, 85, 152 Gouzy, J 37 Kaiser, BN 39, 122, 126 Martínez-Molina, E 154, 155 Borisov, A 65 Díaz-Alcántara, CA 77, 153 Gresshoff, P 57 Kalhor, MS 31 Martinez-Romero, E 66 Braissant, O 41 Díaz-Mínguez, JM 154 Grossman, J 53 Kamaa, M 84 Maseko, S 110 Brau, L 45, 61, 68, 75, 117, Dilworth, M 69 Guerts, R 31, 65 Kamiya, Y 131 Masse, D 109 165, 166 Brock, P 101 Ding, H 35 Hailemariam, A 145 Kaneko, T 131 Mateos, PF 155 Brockwell, J 100 Dinse, T 81 Han, L 125 Kang, H 163 Mathews, C 95, 116 Bruand, C 37 Djedidi, S 33 Hanyu, M 73 Kanu, SA 143 Mathu, S 135 Btissam, M 111 Djordjevic, MA 58 Hao, B 164 Karunakaran, R 36 Matiru, V 135 Bühler, D 41 Dlodlo, O 67 Hara, H 156 Kaur, H 106 Matsuura, H 150 Burgos, FA 24 Kawaharada, Y 51 158 2011
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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />
Fremantle, Western Australia<br />
27 November – 1 December 2011<br />
Title<br />
Selection of plant growth promoting rhizobacteria (PGPR) to enhance nodulation of<br />
grain legumes by rhizobia<br />
Author Liza Parkinson 1 , Graham O‟Hara 1 , Ron Yates 1, 2 , Paul Harvey 3 and Lambert Brau 1<br />
Poster Board Number 51<br />
1 Murdoch University, Centre for Rhizobium Studies, Murdoch, Western Australia, 6150<br />
2 Department of Agriculture and Food Western Australia, South Perth, Western Australia,<br />
6151<br />
3 CSIRO, Urrbrae, South Australia, 5064<br />
Plant growth-promoting rhizobacteria (PGPRs) are soil bacteria associated with plant roots that have been<br />
shown to stimulate plant growth and crop yield. The mechanisms by which PGPRs increase plant growth include<br />
the production of plant hormones, suppression of disease–causing microbes as well as improved plant nutrient<br />
availability and assimilation. It has also been shown that the formation of nodules on legumes induced by<br />
rhizobia can be enhanced by co-inoculation with PGPRs.<br />
To examine the benefits of co-inoculating rhizobia with PGPRs, six newly identified PGPRs will be screened in<br />
glasshouse trials on Lupins (Lupinus angustifolius), Peas (Pisum sativum), Lentils (Lens culinaris), Beans<br />
(Phaseolus vulgaris), Chickpeas (Cicer arietinum) and Soybeans (Glycine max) in addition to the current<br />
commercial rhizobia. PGPRs used in this study include five Pseudomonas species, which have previously been<br />
shown to enhance nodulation in legumes and one gram-positive strain isolated from a Lupinus nodule. To<br />
evaluate the efficacy of the PGPRs on enhancing the legume-rhizobia symbiosis, the following will be measured:<br />
nodule numbers, nodule biomass, shoot and root biomass and shoot nitrogen content. In addition, nodule<br />
occupancy will be analyzed using PCR fingerprinting techniques to establish whether PGPRs are able to colocalize<br />
with rhizobia in the nodules.<br />
Promising isolates from these trails will be selected to elucidate the mechanism by which they enhance<br />
nodulation and/or nitrogen fixation. This will include analysis of expression of putative PGP genes, targeted<br />
gene knock-outs, and population genetic studies on the microbial community to determine the impact of<br />
inoculants on rhizobium populations in the rhizosphere.<br />
166<br />
2011
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