IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

Recommendations

Info

17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Expression of an abscisic acid-responsive ƒÀ-1,3-glucanase gene in autoregulation of nodulation. Author Keni-chi Osuki 1 , Akihiro Suzuki 2 , Hisatoshi Hara 1 , Akihito Takahara 1 , Masato Araragi 1 ,Tadao Asami 3 , Keni-chi Kucho 1 , Shiro Higashi 1 , Mikiko Abe 1 and Toshiki Uchiumi 1 Poster Board Number 41 1 Graduate School of Science and Engineering, Kagoshima University, 2 Department of Environmental Science, Saga University 3 Graduate School of Agriculture and Life Science, The University of Tokyo Host legumes control nodule number by autoregulation of nodulation (AUT), in which the presence of root nodules inhibits further root nodule formation. AUT will consist of at least two long-distance signals, i. e. a rootderived infection signal and a shoot-derived signal that inhibits nodulation. In Lotus japonicus, HAR1 mediates AUT and, LjCLE-RS1 and LjCLE-RS2 have been identified as strong candidates as the infection signal. We employed split-root system of L. japonicus to investigate plant molecules involved in inhibition of nodule formation in AUT. Abscisic acid (ABA) and ABA responsible β-1,3-glucanase gene (designated as LjGlu1) were focused in this study. Plants were set up for split-root system. One side of root systems was inoculated with Mesorhizobium loti MAFF303099. At 1, 2, 3, 5, 7, 9 and 14 days after inoculation, expression of LjGlu1 in leaves, and inoculated- and uninoculated roots, were analyzed by quantitative RT-PCR. In the leaves, expression of LjGlu1 increased at the 7 days after inoculation. In the inoculated- and uninoculated roots, expression of LjGlu1 increased at the 5, 7 and 9 day after inoculation. AUT response of L.japonicus is detectable 3 days after the first inoculation and that full induction of the response occurs after 5 days (Suzuki et al 2008).These results suggest that LjGlu1 increased in the root, when the full induction of the AUT response occurs. Hairy roots transformed with LjCLE-RS1 driven by the Cauliflower mosaic virus 35S promoter were prepared. In the plant that has both the transgenic and untransgenic roots, LjGlu1 was induced in not only the transgenic roots but also untransgenic roots. These findings indicate that constitutive expression of LjCLE-RS1 also induced expression of LjGlu1 systemically. These results suggest that LjGlu1 is functional involved in inhibition of nodule formation in AUT. Akihiro Suzuki , Hisatoshi Hara ,Tomoyo Kinoue ,Mikiko Abe , Toshiki Uchiumi , Ken-ichi Kucho ,Shiro Higashi ,Ann M. Hirsch ,Susumu Arima (2008).Split-root study of autoregulation of nodulation in the model legume Lotus japonicus. J Plant Res 121:245–249 156 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Conservation of legume-rhizobium symbiosis genes in non-legume. Author Keisuke Yokota, Takashi Soyano, Hiroshi Kouchi, Makoto Hayashi Poster Board Number 42 National Institute of Agrobiological Sciences In recent years, a number of legume genes involved in the root nodule symbiosis has been identified in the model legumes, such as Lotus japonicus and Medicago truncatula. Among them, the distinct set of genes has been characterized as common sym, because they are also essential for another mutual interaction, the arbuscular mycorrhiza symbiosis. The non-legume homologs of common sym genes, such as in rice, have been well documented to be not only essential for the arbuscular mycorrhiza symbiosis in non-legume mycorrhizal plants but also functional in both the root nodule symbiosis and the arbuscular mycorrhiza symbiosis in legumes. In contrast, it has not been investigated in detail whether the root nodule symbiosis-specific genes, which are not essential for the arbuscular mycorrhiza symbiosis, are functionally conserved in non-legumes. In this presentation, we will report that the functional conservation analyses of the root nodule symbiosis-specific gene homologs from rice in L. japonicus. References Yokota K, Hayashi M. Function and evolution of nodulation genes in legumes. (2011) Cell Mol Life Sci. 68(8):1341-1351. Yokota K, Soyano T, Kouchi H, Hayashi M. Function of GRAS proteins in root nodule symbiosis is retained in homologs of a non-legume, rice. (2010) Plant Cell Physiol. 51(9):1436-1442. 157 2011
Page 1 and 2:
PrinciPal SPonSor: SPonSorS: Progra
Page 3 and 4:
INVENTING THE FUTURE IN INOCULANTS
Page 5 and 6:
17 ICNF Organising Committee Graham
Page 7 and 8:
Cottesloe Beach © Tourism Western
Page 9 and 10:
GeNeRAl INfoRmATIoN aTM’s Automat
Page 11 and 12:
16:30 - 16:50 Felix Dakora Tshwane
Page 14 and 15:
Thursday 1 December 2011 08:00 - 12
Page 16 and 17:
17 th International Congress on Nit
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107: 17 th International Congress on Nit
show all

IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?