IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

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17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Protection of plant health by Micromonospora isolated from alfalfa root nodules. Authors Pilar Martínez-Hidalgo 1,3 , Pablo García-Benavides 2 ,José Maria Díaz- Mínguez 1 , Ernesto Pérez Benito 1 , Martha E. Trujillo 1, and Eustoquio Martínez-Molina 1 . Poster Board Number 39 1 Departamento de Microbiología y Genética, Universidad de Salamanca 2 Departmento de Construcción y Agronomía, Universidad de Salamanca 3 GIR "Interacciones mutualistas planta microorganismo", Unidad Asociada al IRNASA. CSIC, The studies of new interactions that allow us to design efficient biofertilizers, to reduce the use of pesticides and chemical fertilizers are very important nowadays. The endophytic actinobacteria have a very high biotechnological potential in agriculture and environment. The activity of endophytic actinobacteria can affect (A) the development of plants in a direct way (B) their nutrition, making it easier to get essential elements like nitrogen, iron or phosphore (C) the health of the plants by means of direct mechanisms, like antibiotic or siderophore synthesis or inducing plant defence responses and (D) improving adverse environmental factors tolerance. In this work we study the PGPR effect of actinobacteria from Micromonospora genus that were isolated from alfalfa nodules. Strains that promote plant growth, improve nutrient uptake and nodulation were selected. The selected strains were used, in this work, for studies focused on the protection of plants from pathogens. Protection of plant health by means of direct mechanisms, In vitro studies of growth inhibition of plant pathogens: The following plant pathogens were used for this study: Fusarium circinatum, Sclerotinia sclerotionum, Botrytis cinerea, Rhizoctonia solani, Clavivacter miichiganensis, Erwinia amylovora, Pectobacterium chrisanthemi, Pseudomonas syringae pv. pisi, Pseudomonas syringae pv. syringae, Ralstonia solanacearum, Xanthomonas vesicatoria Micromonospora strains were tested for siderophore production on CAS medium. Protection of plant health by induction of plant defence responses: These studies have been performed on tomato plants using two Micromonospora strains (ALFb5 and ALFpr18c) and two plant pathogens: Botrytis cinerea and Fusarium oxysporum The results obtained point out that the selected strains of Micromonospora may be used to protect plants from pathogens. (i) Some of the tested strains inhibit fungal growth in vitro. (ii) No effect was detected against the tested bacteria. (iii) Many of the strains tested produce siderophores. (iv) Micromonospora induces plant defence response. 154 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Titles Identification by MALDI-TOF MS spectrometry of rhizobia isolated from nodules of different legumes Authors Pilar Martínez-Hidalgo 1 , Laura Ferreira 2 , Martha-Helena Ramírez-Bahena 3 , Lina P. Rivera, Fernando Sánchez-Juanes 2 , Paula García-Fraile 1 , Raúl Rivas 1 , Pedro F. Mateos 1 , José Manuel González-Buitrago 2,4 , Alvaro Peix 3 , Eustoquio Martínez-Molina 1 , , Encarna Velázquez 1 Poster Board Number 40 1 Departamento de Microbiología y Genética, Universidad de Salamanca 2 Unidad de Investigación. Hospital Universitario de Salamanca 3 IRNASA-CSIC. 4 Departamento de Bioquímica y Biología Molecular. Universidad de Salamanca The identification of members from family Rhizobiaceae currently arranged into the genera Rhizobium, Ensifer and Shinella, is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. Therefore alternative methods are necessary for rapid and reliable identification of bacteria isolated from legume nodules. MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization-Time-of- Flight Mass Spectrometry) is a reliable method for bacterial identification, but it was necessary to extend the database Biotyper 2.0 to include the species from family Rhizobiaceae (Ferreira et al., 2011). After database extension, in this work, several strains isolated from nodules of alfalfa, clover and common beans isolated in Spain were subjected to MALDI TOF MS analysis. The results obtained showed a correct identification for most of the strains analysed. Some strains were not identified as any of species from the extended database indicating they can belong to undescribed species. Therefore MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia and detection of new ones applicable to large populations of isolates in ecological and taxonomic studies. Ferreira L, Sánchez-Juanes F, García-Fraile P, Rivas R, Mateos PF, Martínez-Molina E, González-Buitrago JM & Velázquez E (2011) Maldi-tof mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae. PLoS One. 6: e20223. 155 2011
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Cottesloe Beach © Tourism Western
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