IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />
Fremantle, Western Australia<br />
27 November – 1 December 2011<br />
Title BNF efficiency of rhizobia isolates from Phaseolus vulgaris in subsistence agrosystems in<br />
Dominican Republic.<br />
Authors César A. Díaz-Alcántara 1 , Beatriz Urbano 2 , Daniel Mulas 3 , Encarna Velázquez 4 , Fernando<br />
González-Andrés 3 .<br />
Poster Board Number 38<br />
1 Facultad de CC. Agronómicas y Veterinarias. Univ. Autónoma de Santo Domingo.<br />
Dominican Republic.<br />
2 Departamento de Ingeniería Agrícola y Forestal. Universidad de Valladolid. Spain.<br />
3 Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad. Universidad de León.<br />
Spain.<br />
4 Departamento de Microbiología y Genética. Universidad de Salamanca. Spain.<br />
Biofertilisation of common bean (Phaseolus vulgaris L.) using selected native rhizobial strains can improve the crop yield in<br />
subsistence agrosystems of Dominican Republic. With this objective 5 rhizobial strains were preselected in the basis of N<br />
fixation efficiency in axenic conditions, from an initial collection consisting of 23 strains isolated from root nodules of<br />
common bean (Phaseolus vulgaris L.) growing in subsitence agrosystems in 3 provinces of Dominican Republic (Elías Piña,<br />
San José de Ocoa and La Vega). On the basis of the 16S rRNA and housekeeping genes (recA and atpD) sequence<br />
analysis, the 5 strains belonged to the Rhizobium genus, although the identity with the previously described species is too<br />
low to precisely identify them, so they could be new species, which needs further research (Díaz-Alcántara et al., 2008). In<br />
order to assess the potential benefit of the inoculation with the preselected strains for the farmers, a microcosm experiment<br />
was carried out in 3 different soils form Elías Piña, San José de Ocoa and La Vega respectively, with the purpose of<br />
compare the effect of inoculation vs. the conventional N fertilisation. In spite of the limiting conditions of microcosm for<br />
nodulation and BNF because of the high temperatures inside the pots, 2 out of the 5 strains produced significantly higher<br />
aerial biomass than the non fertilised control. The interaction between soil and strain was not significant for p