IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

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17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Rhizobium delivery systems for grain legumes in southern Australia Author David Pearce 1 , Bernadette Carmody 1 , Matthew Denton 2 , Mark Peoples 3 , Lori Phillips 4 Poster Board Number 37 1 Department of Primary Industries 2 University of Adelaide 3 CSIRO Plant Industry, Canberra 4 Department of Primary Industries Grain legumes are an integral component in Australian cropping systems. Due to low soil Rhizobium populations, inoculation is often required to provide sufficient numbers of suitable rhizobia on or near the germinating seed. Traditionally, the main inoculation delivery system has been peat slurry application, where the seed is coated with the appropriate rhizobia just prior to sowing. Although very effective, this method requires strict adherence to application recommendations and may be too troublesome for growers during sowing. New inoculation delivery technologies are much easier to use and have the potential to increase inoculation flexibility at sowing. However, there is still a need for field evaluation to ensure that optimal nodulation, crop performance, and nitrogen (N) fixation occurs. In this study, we evaluated the performance of different delivery systems in Southern New South Wales. Faba bean cv. Farah and Lupin cv. Jindalee were sown using three delivery systems (peat slurry on seed, peat granules, peat slurry injection) and non-inoculated controls. Performance was measured with respect to nodulation, herbage biomass, grain yield, N content, and total N fixed. Inoculation with any method was significantly better than no inoculation. The peat slurry on seed method also generally outperformed the other methods. For example, Faba inoculated with this method yielded 3.69 t/ha of grain (4.37% N) and 11.61 t/ha of herbage (2.79% N). In comparison, Faba inoculated using peat slurry injection yielded 2.70 t/ha of grain (4.03% N) and 8.40 t/ha of herbage (2.48% N). Greater crop biomass with higher N content resulted in increased N fixed within the cropping system as a whole. Peat-seed inoculation systems fixed 316 kgN/ha, while peat-injection systems fixed 188 kgN/ha. Our results highlight not only the value of inoculation at sowing, but also the importance of choosing the appropriate inoculation delivery system for local conditions. 152 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title BNF efficiency of rhizobia isolates from Phaseolus vulgaris in subsistence agrosystems in Dominican Republic. Authors César A. Díaz-Alcántara 1 , Beatriz Urbano 2 , Daniel Mulas 3 , Encarna Velázquez 4 , Fernando González-Andrés 3 . Poster Board Number 38 1 Facultad de CC. Agronómicas y Veterinarias. Univ. Autónoma de Santo Domingo. Dominican Republic. 2 Departamento de Ingeniería Agrícola y Forestal. Universidad de Valladolid. Spain. 3 Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad. Universidad de León. Spain. 4 Departamento de Microbiología y Genética. Universidad de Salamanca. Spain. Biofertilisation of common bean (Phaseolus vulgaris L.) using selected native rhizobial strains can improve the crop yield in subsistence agrosystems of Dominican Republic. With this objective 5 rhizobial strains were preselected in the basis of N fixation efficiency in axenic conditions, from an initial collection consisting of 23 strains isolated from root nodules of common bean (Phaseolus vulgaris L.) growing in subsitence agrosystems in 3 provinces of Dominican Republic (Elías Piña, San José de Ocoa and La Vega). On the basis of the 16S rRNA and housekeeping genes (recA and atpD) sequence analysis, the 5 strains belonged to the Rhizobium genus, although the identity with the previously described species is too low to precisely identify them, so they could be new species, which needs further research (Díaz-Alcántara et al., 2008). In order to assess the potential benefit of the inoculation with the preselected strains for the farmers, a microcosm experiment was carried out in 3 different soils form Elías Piña, San José de Ocoa and La Vega respectively, with the purpose of compare the effect of inoculation vs. the conventional N fertilisation. In spite of the limiting conditions of microcosm for nodulation and BNF because of the high temperatures inside the pots, 2 out of the 5 strains produced significantly higher aerial biomass than the non fertilised control. The interaction between soil and strain was not significant for p
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