IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

Recommendations

Info

17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Phylogenetic position of Rhizobium galegae Authors Seyed Abdollah Mousavi 1 , Janina Österman 1 , Lars Paulin 2 & Kristina Lindström 1 Poster Board Number 31 1 Department of Food and Environmental Sciences 2 Institute of Biotechnology Rhizobia are defined as bacteria that can enter nitrogen-fixing symbioses by inducing nodules in legumes (Lindström & Mousavi 2010), whilst agrobacteria induce tumours in plants. Rhizobium galegae is fixing nitrogen in Galega plants, and is one of the species of the family Rhizobiaceae. The phylogenetic placement of R. galegae is not stable in phylogenetic trees of rhizobia and it does not fit with most other rhizobia in some gene trees. R. galegae was e.g. placed in the Rhizobium rhizogenes - Rhizobium cluster in dnaK nucleotide sequence trees; whereas it was placed with Agrobacterium species in the 16S rRNA tree (Eradly at al 2005). Construction ofa concatenated tree of eight housekeeping genes, recA, dnaK, thrC, gltA, rpoB, atpD, pnp and gyrB, for twenty whole-genome sequenced rhizobia and agrobacteria showed that R. galegae could be placed in a cluster with A. vitis and A. tumefaciens, whereas Rhizobium rhizogenes K84 (formerly A. radiobacter K84) was situated in the Rhizbium clade, wihch included R. etli and R. leguminosarum. To get a robust estimate of the phylogenetic placement of R. galegae, four house-keeping genes (recA, thrC, atpD and rpoB), and glnA and glnII genes of forty-four strains of R. galegae and more than forty strains of related rhizobia and agrobacteria were sequenced. Results of Multilocus Sequence Analysis of rhizobia and agrobacteria will be presented. Eardly BD, Nour SM, van Berkum P & Selander RK (2005) Rhizobial 16S rRNA and dnaK genes: Mosaicism and the Uncertain Phylogenetic Placement of Rhizobium galegae, Appl Environ Microbiol 71: 1328-1335. Lindström K & Mousavi SA (2010) Rhizobium and Other N-fixing Symbioses. In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester. DOI: 10.1002/9780470015902.a0021157. 146 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Study on soybean nodule symbiosome membrane localised transporters Authors Chen C., Loughlin P., Day D., Smith P. Poster Board Number 32 In nodules, the symbiosome membrane (SM) shares properties of both the plasma membrane and vacuolar membrane, however the unique nature of the SM suggests specific mechanism(s) for targeting proteins is essential. Recent work has indentified the first 24 amino acids from the N-terminus of a Medicago symbiosome protein as an address label (signal peptide) for its targeting (Hohjnec et al., 2009), however this signal peptide is not present on many known SM proteins. We are focusing on clarifying the targeting mechanism of soybean nodule SM-localised GmZIP1 (zinc transporter) and GmYSL1 (iron transporter). In silico analysis predicts the first 28 amino acids of GmZIP1 may be a signal peptide. Full length GmZIP1 with GFP fused internally, immediately after the last predicted transmembrane domain is targeted to the SM. Deletion of the predicted signal peptide appears to disrupt the expression and/or localization of the fusion protein. GmYSL1 is not predicted to have an N-terminal signal peptide and N-terminal fusion of GFP does not disrupt its SM localisation. We are currently examining the localisation of a C-terminally truncated GmYSL1 construct to determine whether this is important for SM targeting. Furthermore we have characterised 4 novel soybean promoters that can drive high and specific expression in soybean nodule infected cells. Role of other soybean ZIP homologues are being characterised through expression, function and localisation studies to elucidate the zinc transporter family. 147 2011
Page 1 and 2:
PrinciPal SPonSor: SPonSorS: Progra
Page 3 and 4:
INVENTING THE FUTURE IN INOCULANTS
Page 5 and 6:
17 ICNF Organising Committee Graham
Page 7 and 8:
Cottesloe Beach © Tourism Western
Page 9 and 10:
GeNeRAl INfoRmATIoN aTM’s Automat
Page 11 and 12:
16:30 - 16:50 Felix Dakora Tshwane
Page 14 and 15:
Thursday 1 December 2011 08:00 - 12
Page 16 and 17:
17 th International Congress on Nit
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97: 17 th International Congress on Nit
show all

IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?