IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

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17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Isolation and characterization of diazotrophic microsymbionts from root nodules of mucuna bracteata Authors Salwani Shaffie ,Amir Ghazali, and Nazalan Najimudin Poster Board Number 21 School of Biological Sciences, Universiti Sains Malaysia An increasing number of bacteria that can nodulate and fix N2 in legumes which do not belong to the genus of Rhizobium or Bradyrhizobium was identified. This study was conducted to determine indigenous microsymbiont strains which could further promote symbiotic N2-fixation activities for Mucuna bracteata (an important leguminous cover crop) and to verify the identity of the isolates based on nitrogenase enzyme assay, nifH gene isolation and partial 16S rDNA sequence analysis. Our findings indicated that the isolated microsymbionts could nodulate and promote N2-fixation activity in M. bracteata. The isolates also contributed to enhanced plant growth in terms of leaf protein and chlorophyll content and higher plants and nodule biomass. Additionally, the nifH gene fragments were successfully amplified for all of the isolates. The 16S rDNA sequencing results suggested that bacteria which were able to form N2-fixing symbioses with root known as rhizobia is not only from the α-class of proteobacteria but also from β-class of proteobacteria (Burkholderia sp. and Achromobacter sp., 99-100% of similarity) and γ-class of proteobacteria (Stenotrophomonas sp., 99% of similarity). The findings indicate the diversity of potentially-beneficial diazotrophic microsymbionts active in this emerging legume species. 136 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Lipopolysaccharide binding protein, a new requirement for legume-rhizobium symbiosis Author Ei-ichi Murakami 1 , Hitomi Takayama 1 , Ken-ichi Osuki 1 , Maki Nagata 1 , Yoshina Hori 2 , Mayumi Shigeoka 2 , Mana Sahara 2 , Yoshikazu Shimoda 3 Sayaka Muto 4 , Yukio Nagano 4 , Ken-ichi Kucho 1 , Mikiko Abe 1 , Shusei Sato 5 , Shiro Higashi 2 , Toshiki Uchiumi 1 Poster Board Number 22 1 Graduate School of Science and Engineering, Kagoshima University, Kagoshima, Japan. 2 Faculty of Science, Kagoshima University, Kagoshima, Japan. 3 National Institute of Agrobiological Sciences, Ibaraki, Japan. 4 The United Graduate School of Agricultural Sciences, Kagoshima University, Kaogoshima, Japan. 5 Kazusa DNA Research Institute, Chiba, Japan Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria. LPS induces the plant resistance in pathogenic combination and is also involved in the establishment of symbiosis between legumes and rhizobia. Thus the elucidation of LPS recognition system is essential for understanding the plantbacteria interaction. In mammals, LPS binding protein (LBP) has already been identified and signal transduction system via Toll-like receptor TLR4 is well investigated. However, LPS recognition system is still unknown in plants. Referring LBP of mammals, four LBP-candidates, LjLBP1, LjLBP2, LjLBP3 and LjLBP4, were identified on the genome of Lotus japonicus. N-terminal barrel of LjLBP1, LjLBP2 and LjLBP3 bound with LPS of Escherichia coli. The expression levels of LjLBP1 and LjLBP2 were low and stable, whereas LjLBP3/4 was inducible in responding to rhizobial inoculation. In the nodules on the hairy roots of LjLBP1-RNAi, nodule cells were broken and no symbiosome was observed. The shape of bacteroids of LjLBP2-RNAi was abnormal. Symbiosomes of LjLBP3/4-RNAi were larger than those of the control. These results suggest that LjLBPs are required for the symbiosis between L. japonicus and M. loti. 137 2011
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PrinciPal SPonSor: SPonSorS: Progra
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INVENTING THE FUTURE IN INOCULANTS
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17 ICNF Organising Committee Graham
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Cottesloe Beach © Tourism Western
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GeNeRAl INfoRmATIoN aTM’s Automat
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16:30 - 16:50 Felix Dakora Tshwane
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