IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />
Fremantle, Western Australia<br />
27 November – 1 December 2011<br />
Title Efficiency of Bradyrhizobium japonicum in different formulations and when co-inoculated with<br />
bacillus subtilis on soybean in a Kenyan soil<br />
Authors Mary Atieno 1, 2 , Laetitia Herrmann 2 , Robert Okalebo1 , Didier Lesueur 2, 3<br />
Poster Board Number 19<br />
1 Moi University, Department of Soil Science, PO Box 1125-30100, Eldoret, Kenya<br />
2 Tropical Soil Biology and Fertility Institute of the <strong>International</strong> Centre for Tropical Agriculture<br />
(TSBF-CIAT), United Nations Avenue, PO Box 30677-00100, Nairobi, Kenya<br />
3 CIRAD, UMR Eco&Sols (CIRAD-IRD-<strong>IN</strong>RA-SupAgro), Land Development Department,<br />
2003/61 Paholyothin Road, Lardyao Chatuchak, Bangkok 10900 Thailand<br />
The major setback in successfully obtaining an effective inoculant is overcoming difficulties in formulating a<br />
viable and user-friendly final product as the live nature of the active ingredient underscores the importance of<br />
formulation in maintaining the microbial cells in a competent state. Co-cultures of rhizobia and PGPR (Plant<br />
Growth Promoting Rhizobacteria) are also known to influence the efficacy of the symbiotic bacteria on plant<br />
biological nitrogen fixation. A greenhouse experiment was set to assess the formulation effect of one strain i.e.<br />
Bradyrhizobium japonicum, 532c (granules, liquid and broth) and to determine the efficiency of co-inoculation of<br />
Bacillus subtilis with two strains of Bradyrhizobium japonicum (532c and RCR 3407). The objectives were<br />
evaluated on 2 soybean (Glycine max L.) varieties: Nyala, a non-promiscuous variety and TGx1740-2F, a<br />
promiscuous test variety. A non sterile soil from Central Kenya (Chuka) classified as a Nitisol was used. Nodule<br />
occupancy was determined by PCR-RFLP. Most of the inoculants showed increased nodulation and biomass<br />
yields as compared to the un-inoculated controls with a higher response seen in the promiscuous TGx1740-2F<br />
variety as compared to the non-promiscuous variety. The liquid and granule-based inoculants had higher<br />
biomass yields suggesting an impact of formulation on the effectiveness of the inoculants. The co-inoculants also<br />
gave higher yields but showed no significant differences to the rhizobial inoculants alone. Nodule occupancy was<br />
100 % for all the rhizobial inoculants as well as the co-inoculants emphasizing the infectivity and high<br />
competitiveness of 532c and RCR 3407 strains even in the presence of indigenous strains (80-113 cell/g of soil).<br />
These inoculants, though not initially made for SSA countries, showed promising increased yields in a Kenyan<br />
soil containing significant populations of native rhizobia nodulating soybean, signifying a possibility of their<br />
adoption in increasing soil fertility and crop yields in the poor SSA soils.<br />
134<br />
2011