IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...

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17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Title Rice growth promotion after inoculation of Sinorhizobium melilotii 1021 and its mechanism Authors Mingfeng Yang, Wenpeng Zhang, Shihua Shen and Yuxiang Jing Poster Board Number 9 Key Laboratory of Plant Resource Research and Development, the Chinese Academy of Sciences, Beijing 100093, China. The studies have shown that the endophytic association of rhizobia with non-legumes, such as rice, wheat, maize, rape, lettuce, etc is of benefit to their growth with increased grains or/and biomass. Our previous study indicated that the bacteria of Sinorhizobium melilotii 1021, etc which were inoculated to rice seedlings not only entered into root interior through lateral root emergence and root hairs, but also ascended into stem base, leave shealth and leaves where they developed high population. Finally, they made rice increase its biomass and grains (Chi F. et al, 2005). Recently, we found the key point time that the rice seedlings inoculated with rhizobia grew more significantly than the controls based on rice morphology was at 5 days postinoculation(dpi). The observation under laser confocal microscope pointed out that the gfp-tagged rhizobia moved so fast that they arrived in rice leaves at the 5-8 dpi, and located in the intercellular space, and some of them even got into diachyma cells after isolation of leaf cell protoplasm. Microarray-gene chip and quantitative real-time PCR determined the gene expression of rice seedlings, showing that the related genes of DNA replication, cell division and cell wall formation, etc were up-reguated at 2 dpi, then some of photosynthetic genes, carbohydrate anabolism genes, defense and resistant genes were also up-regulated at 5, 8 dpi. These results indicate the molecular basis and the mechanism that the rhizobia could promote rice growth. The following step in future is to look for the reasons of molecular interaction between rhizobia and rice plants through metabolomics. Feng Chi, Shi-Hua Shen, Hai-Ping Cheng, Yu-Xiang Jing,* Youssef G. Yanni, and Frank B. Dazzo. Ascending Migration of Endophytic Rhizobia, from Roots to Leaves, inside Rice Plants and Assessment of Benefits to Rice Growth Physiology. AEM, 2005, 71: 7271–7278. 124 2011
17 th International Congress on Nitrogen Fixation Fremantle, Western Australia 27 November – 1 December 2011 Titles Differential activation of CCAMK between root nodule and arbuscular mycorrhizal symbioses Authors Yoshikazu Shimoda 1 , Lu Han 1 , Makoto Hayashi 1 , Haruko Imaizumi-Anraku 1 Poster Board Number 10 1 National Institute of Agrobiological Sciences, Division of Plant Sciences, Tsukuba, Ibaraki 305-8602, Japan Leguminous plants can form mutually beneficial endosymbioses with rhizobial bacteria and arbuscular mycorrhizal (AM) fungi. Recent genetic studies in model legumes have revealed that the two symbiotic systems share a common signaling pathway (CSP), which is essential for the induction of cytosolic Ca 2+ spiking in response to infection signal molecules derived from rhizobia or AM fungi. Among the components of CSP, Ca 2+ /calmodulin(CaM)-dependent protein kinase (CCaMK) is downstream of Ca 2+ spiking and thought to be an ideal decoder for microsymbionts-induced Ca 2+ signals, because of its domain structure including CaM binding domain and EF hand motifs. Recent studies have identified crucial roles of CCaMK for bacterial/fungal infections and nodule organogenesis. However, it remains unknown how CCaMK is activated differentially in response to Ca 2+ signals induced by rhizobia or AM fungi. In order to elucidate the difference of CCaMK activation mechanisms during RN and AM symbioses, we carried out a detailed complementation analysis of ccamk mutant of Lotus japonicus with various kinds of mutated CCaMKs. We also analyzed epistatic relationships among CCaMK functional domains by combining the mutations of each domain to clarify the involvement of the domains in Ca 2+ -dependent activation of CCaMK. In this presentation, we propose an activation mechanism of CCaMK in which RN and AM symbioses are distinguished by differential regulation of CCaMK by Ca 2+ signals. 125 2011
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INVENTING THE FUTURE IN INOCULANTS
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17 ICNF Organising Committee Graham
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Cottesloe Beach © Tourism Western
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GeNeRAl INfoRmATIoN aTM’s Automat
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16:30 - 16:50 Felix Dakora Tshwane
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