IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
IN INOCULANTS Nodulaid - 17th International Nitrogen Fixation ...
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17 th <strong>International</strong> Congress on <strong>Nitrogen</strong> <strong>Fixation</strong><br />
Fremantle, Western Australia<br />
27 November – 1 December 2011<br />
Session Details: Thursday 1 December 2011<br />
Authors:<br />
Concurrent Session 16 – Symbiotic Impacts & Emissions<br />
1100 - 1230<br />
Kiwamu Minamisawa, Manabu Itakura, Shoko Inaba, and Yoko Shiina.<br />
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aobaku, Sendai,<br />
980-8577, Japan<br />
Presentation Title: Mechanism of N2O emission from soybean nodule rhizosphere and its mitigation<br />
Presentation Time: 1140 – 1200<br />
Nitrous oxide (N2O) is a greenhouse gas that also degrades stratosphere ozone. Agricultural land is a major<br />
source of N2O through microbial transformation of nitrogen in plant-soil ecosystems. In particular, more N2O is<br />
emitted from legumes than that with non-legumes. Marked N2O emission were detected from root systems with<br />
degraded nodules during late growth stage in field-grown soybeans. To clarify the mechanism of N2O emission, a<br />
model system was developed in laboratory. Thirty-days after soybean plants inoculated with B. japonicum were<br />
cultivated in Leonard‟s jars, treatments of shoot decapitation (D) and/or soil addition (S) were conducted to<br />
simulate the nodule degradation and subsequent N2O emission. Double treatment (DS) resulted in the<br />
degradation and N2O emission from the nodules formed with B. japonicum lacking nosZ. These results<br />
suggested that soil microbes are required for the N2O emission from degraded soybean nodules. To evaluate<br />
bradyrhizobial contribution, N2O emission was compared between nirK mutant (∆nirK) and wild-type USDA110<br />
under identical nosZ genetic backgrounds. N2O emission from the nodules formed with ∆nirK∆nosZ mutant was<br />
significantly lower than that from ∆nosZ mutant under DS treatment, but retained approximately a half amount of<br />
N2O emission in ∆nosZ mutant (30-60%), suggesting that nitrate reduction to N2O is due to both B. japonicum<br />
and other soil microorganisms. On the other hand, it is likely N2O reduction to N2 was mainly mediated by B.<br />
japonicum cells carrying nosZ, which was consistently supported by the comparisons between wild-type<br />
USDA110 and nosZ mutants. Thus, B. japonicum plays an important role in determining N2O flux from soybean<br />
rhizosphere as well as unknown soil microorganisms. To mitigate N2O emission from soybean rhizosphere, we<br />
developed B. japonicum mutants with higher N2OR activity, and inoculated them to field soils where nosZ minus<br />
strains of B. japonicum are dominant. As a result, N2O emission from soybean rhizosphere significantly reduced<br />
in Leonard‟s jar system.<br />
102<br />
2011