PeloBiotech
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www.pelobiotech.com<br />
light source, and its emission is transferred to the acceptor fluorophore (e.g., XL665 or APC), resulting in a FRET<br />
signal.<br />
• Target Molecules: These are the biological components under investigation, such as proteins, nucleic acids, or<br />
small molecules, labeled with the donor or acceptor fluorophores. The choice of labeling strategy depends on the<br />
specific experiment and the accessibility of the target sites.<br />
• Buffer Solutions: Appropriate buffer solutions are used to maintain physiological conditions and optimize the<br />
stability of the labeled molecules.<br />
• Time-Resolved Detection System: This includes a fluorescence plate reader or a dedicated TR-FRET reader<br />
equipped with the capability to measure the time delay between excitation and emission of the fluorophores.<br />
TR-FRET has revolutionized the field of molecular biology and drug discovery by enabling the study of complex cellular<br />
processes in a high-throughput and sensitive manner. Researchers can use TR-FRET to assess biomolecular interactions,<br />
screen for potential drug candidates, and gain insights into cellular pathways and mechanisms with exceptional accuracy<br />
and efficiency.<br />
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