PeloBiotech

www.pelobiotech.com
Nucleic Acid and Telomere Length Assays
• Absolute and Relative Telomere Length Assay Kits: These kits are vital for measuring telomere length, which is linked
to aging and various diseases, including cancer and cardiovascular conditions.
• qRT-PCR Kits: These quantitative reverse transcription-polymerase chain reaction kits are used to analyze gene
expression and are crucial for studying genes involved in cell cycle control and senescence.
Metabolic and Redox Assays
• Biopyrrin ELISA kit, Redox Assay: This assay quantifies biopyrrin, a marker for oxidative stress. It's applied to study
oxidative damage in various biological systems.
• Lactate Dehydrogenase (LDH) Assay: LDH is an enzyme released during cell damage. This assay helps assess cellular
cytotoxicity and damage, often used in cytotoxicity studies and assessing cell health.
• Creatinine, Urinary, ELISA Kit: Used to measure urinary creatinine levels, this assay aids in evaluating kidney function
and diagnosing renal diseases.
Cell Viability and Proliferation Assays
• MTT and WST-1 Cell Viability & Proliferation Assays: These assays assess cell viability, metabolic activity, and
proliferation. They are employed in drug screening, cytotoxicity testing, and assessing the effects of various
compounds on cell health.
TR-FRET
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a powerful and versatile molecular assay technique
used in biological research and drug discovery. TR-FRET allows scientists to study molecular interactions and events within
cells or biochemical reactions with high sensitivity and precision. It is particularly valuable for measuring protein-protein
interactions, receptor-ligand binding, and various other biomolecular processes.
TR-FRET relies on the principles of fluorescence resonance energy transfer (FRET), in which energy is transferred from one
fluorophore (the donor) to another (the acceptor) when they are in close proximity, typically within 10-100 angstroms. The
key distinction in TR-FRET is the measurement of the time delay between excitation and emission, which eliminates shortlived
background fluorescence and significantly enhances signal-to-noise ratios.
To perform TR-FRET assays, researchers require specific reagents:
• Donor and Acceptor Fluorophores: These are the fluorescent molecules that emit light when excited by an energy
source. The donor fluorophore, typically a lanthanide chelate (e.g., Europium or Terbium), is excited by a pulsed
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