PeloBiotech

www.pelobiotech.com
Cell-based Assays (ECM Assays)
Cell biology assays are pivotal in the field of life sciences, enabling researchers to investigate and understand various cellular
processes. Among the essential tools are the Cyto-X Solution, available in both full kits and sample sizes, which provide a
colorimetric method for precise cell counting.
For researchers interested in the extracellular matrix (ECM) and its influence on cell behavior, the MicroMatrix 36 and
MicroMatrix 36-4 Pack are ideal for testing the most suitable ECM for specific cell types. The screenMatrix in a 96-well
format facilitates high-throughput screening, making it a valuable tool for cell biology studies. These assays collectively
contribute to our understanding of cellular functions and the optimization of cell culture conditions.
Cell Purity Kits
Accumol's MTBN Purity Assessment Kit is a versatile tool for detecting Monocyte, T-Cell, B-Cell, and Neutrophil mRNA in
genomic DNA samples isolated from sorted cells. This kit is specifically designed to quantify lineage-specific RNAs that are
co-purified during standard DNA isolation procedures. After reverse transcription and PCR amplification, the resulting
products can be analyzed on a sequencer or traditional gels. This test is not influenced by the presence of beads or antibodies
on sorted cells' surfaces and can be applied regardless of the sorting method used. Furthermore, as it assesses purity at the
nucleic acid level, it allows for delayed analysis, even months after cell sorting.
Chimerism Assays
Checking chimerism, the presence of two or more genetically distinct cell populations within an individual, is crucial for a
variety of applications in the field of medicine and research. The provided Non-B Genomic Detection Kits and Non-T
Genomic Detection Kits, as well as the qPCR Non-Myeloid Genomic Detection Kit, offer powerful tools for this purpose.
Chimerism testing plays a vital role in clinical contexts, such as post-transplant monitoring, where assessing the proportion
of donor and recipient cells is essential to evaluate the success of organ or bone marrow transplants. It is also instrumental
in identifying and tracking the presence of foreign cells in cases of graft-versus-host disease and determining the
effectiveness of therapeutic interventions.
In forensic science, chimerism testing is valuable for solving complex cases by distinguishing between the DNA profiles of
individuals. In research, chimerism analysis helps scientists understand developmental processes, genetic mosaicism, and
the dynamics of cell populations within organisms.
These detection kits are indispensable in these applications, providing accurate and reliable methods to assess chimerism,
ultimately contributing to improved patient care, the advancement of forensic investigations, and a deeper understanding
of biological mechanisms.
Metallo-quantification Assays
Metallo quantification assays, such as the Copper, Iron, Magnesium, and Zinc Assay kits provided, are essential tools in
analytical chemistry and various scientific disciplines. These kits offer precise methods for quantifying the concentration of
specific metal ions in various samples, employing different detection techniques like the 3,5-DiBr-PAESA, Ferrozine, Nitroso-
PSAP, Xylidyl Blue-I, and 5-Br-PAPS methods.
In the field of clinical and biomedical research, metallo quantification is important for understanding the role of metal ions
in biological processes, such as iron in hemoglobin or copper in enzymes. Monitoring metal ion concentrations is also vital
for diagnosing and managing various diseases, including iron-deficiency anemia and Wilson's disease.
Metastasis Assays
Metastasis is a critical process in cancer biology, where cancer cells spread from the primary tumor to other parts of the
body, often leading to advanced disease and poor patient outcomes. The IN SITE Metastasis Hydrogel Kit and Metastasis
Surface Coating Kit are instrumental tools in studying and understanding this complex phenomenon. By recreating a
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