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diseases. G-CSF is crucial for recovering white blood cell counts after chemotherapy. IFNs like interferon-beta have
antiviral and anti-inflammatory applications. Animal-free versions cater to applications where the absence of
animal-derived components is critical, ensuring ethical and controlled use. This distinguishes them from cGMPproduced
cytokines. Reach out to us for specific details tailored to meet your research needs.
TR-FRET
Time-Resolved Fluorescence Resonance Energy Transfer
(TR-FRET) is a powerful and versatile molecular assay
technique used in biological research and drug discovery.
TR-FRET allows scientists to study molecular interactions
and events within cells or biochemical reactions with high
sensitivity and precision. It is particularly valuable for
measuring protein-protein interactions, receptor-ligand
binding, and various other biomolecular processes.
TR-FRET relies on the principles of fluorescence resonance
energy transfer (FRET), in which energy is transferred from
one fluorophore (the donor) to another (the acceptor)
when they are in close proximity, typically within 10-100
angstroms. The key distinction in TR-FRET is the measurement of the time delay between excitation and emission, which
eliminates short-lived background fluorescence and significantly enhances signal-to-noise ratios.
To perform TR-FRET assays, researchers require specific reagents:
• Donor and Acceptor Fluorophores: These are the fluorescent molecules that emit light when excited by an energy
source. The donor fluorophore, typically a lanthanide chelate (e.g., Europium or Terbium), is excited by a pulsed
light source, and its emission is transferred to the acceptor fluorophore (e.g., XL665 or APC), resulting in a FRET
signal.
• Target Molecules: These are the biological components under investigation, such as proteins, nucleic acids, or
small molecules, labeled with the donor or acceptor fluorophores. The choice of labeling strategy depends on the
specific experiment and the accessibility of the target sites.
• Buffer Solutions: Appropriate buffer solutions are used to maintain physiological conditions and optimize the
stability of the labeled molecules.
• Time-Resolved Detection System: This includes a fluorescence plate reader or a dedicated TR-FRET reader
equipped with the capability to measure the time delay between excitation and emission of the fluorophores.
TR-FRET has revolutionized the field of molecular biology and drug discovery by enabling the study of complex cellular
processes in a high-throughput and sensitive manner. Researchers can use TR-FRET to assess biomolecular interactions,
screen for potential drug candidates, and gain insights into cellular pathways and mechanisms with exceptional accuracy
and efficiency.
Europium chelates
Europium chelates are a class of luminescent compounds widely used in various applications, particularly in the field of
fluorescence-based assays and diagnostics. These chelates consist of europium ions (Eu3+) bound to organic chelating
ligands, which enhance their stability and luminescent properties. Europium ions are known for their unique photophysical
properties, including a long fluorescence lifetime, narrow emission bands, and resistance to photobleaching. These features
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