PeloBiotech

www.pelobiotech.com
Benefits of using PerfusionPAL :
• Superior oxygenation and gas exchange
• Physiologically relevant interstitial perfusion
• Long-term culturing, testing and analysis
• In-well assaying (supernatants can be transferred to another plate for reads)
• Time-course analyses by sampling secretome and metabolome from wells, using ordinary micropipette
• Customizable perfusion rates
• Reliable perfusion (there are no bubbles and no microchannels to clog)
• Statistically independent wells
• Zero dead volume in fluidics
• Sterile and ready to use
• Blood Substitute can be reused, and maintains purity
• Blood Substitute is inert and immiscible with media, reagents, drugs etc.
• Offers more precision and control compared to animal models
GradientEZ
GradientEZ is a qualitative cell migration and invasion assay that enables cells to migrate in three dimensions as
they do in living tissues. It is straightforward to use and works like a piece of paper. Simply, pipet cells in the center
and add test agents to petals or lobes to model physiologically relevant, gradient-dependent pathological
conditions in 3D. Alternatively, add invasive cancer cells to the center and normal cells to lobes. Fluorescently labeled
cells or cell populations added to the center or lobes may be imaged and analyzed in real time during or after cell
migration studies using fluorescence microscopy.
Suitable for both adherent and suspension cells.
How does it work?
Method 1:
Migrating or invading cells, and
different cell populations or test
compounds may be added to
the center and 6 petals or lobes
Method 2:
To test metastatic potential of cancer
cells in vitro, add invasive cells to the
center and normal cells (e.g. brain,
lung, liver, bone, adrenal, etc.) to lobes
and track invasion to begin to elucidate
where they might
metastasize in vivo.
Benefits of using GradientEZ:
• Closely mimics physiologically relevant in vivo conditions
• Fits in any Petri dish, 6-well to 12-well plates & 6-well inserts
• Test modulators of cell motility or invasion, in parallel, in one assay
• Up to six different concentrations of the same agent can be tested in one well
Applications:
• Cell migration assays, cell invasion assays, chemo-invasion assays, chemotaxis assays
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