PeloBiotech
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www.pelobiotech.com<br />
PerfusionPAL<br />
What is Perfusion?<br />
Perfusion describes the rate at which blood is supplied to a given organ<br />
in the body. We believe this crucial factor can play a role in cell culture<br />
methods in order to positively impact cell survival mechanisms in vitro<br />
as well as simulate real life growth conditions for your research.<br />
Perfusion delivers oxygen to regulate metabolic activity of cells and<br />
promotes dissipation of heat. Poor perfusion may lead to cell death,<br />
and on a larger scale tissue necrosis. Our technology can be adjusted<br />
to deliver the specific perfusion rate needed for your model.<br />
What is Perfused Organ Panel?<br />
The POP system is a lab technology that combines the 3D cell<br />
culturing capabilities of the SeedEZ scaffold with the advanced<br />
oxygenation of our Blood Substitute within a compact, perfused<br />
multi-well plate, PerfusionPal. The system is ideally suited for<br />
modeling interstitial perfusion that cells in most tissues experience in<br />
vivo.<br />
Our PerfusionPal plate is easily perfused with a single tube that is<br />
attached to the base of the plate. The tube is flexible and has a durable<br />
metal sheath that you can use to route the tubing between the gasket<br />
and the door of the incubator. At the other end of the tubing is a syringe<br />
pump, with room for 2 syringes to operate 2 PerfusionPal plates at<br />
once.<br />
The perfusion rate is set by the user and can be easily paused for<br />
alterations, or stopped for media changes. Pumping of this blood<br />
substitute acts as a "liquid piston" that introduces perfusion<br />
uniformly along the plate, without the need for separate pumps and<br />
tubing for each culture.<br />
pics. Lena Biosciences<br />
Applications include:<br />
• Small molecule drug testing: drug metabolism, on-target efficacy & off-target toxicity<br />
• Large molecule biopharmaceutical testing: interstitial delivery mimicking in vivo conditions, on-target efficacy/ off-target<br />
toxicity of antibody-drug-conjugates & multi-specific antibodies<br />
• Cancer research: simulate advanced, vascularized tumors with heterogeneous cell populations, extracellular matrix, & intratumor<br />
supply of drugs to more closely mimic a patient’s tumor at the time of treatment<br />
How does it work?<br />
Step 1:<br />
Add Blood Substitute to<br />
the bottom of the<br />
PerfusionPal tray<br />
Step 2:<br />
Insert middle component<br />
containing wells, and add<br />
media to each well<br />
Step 3:<br />
Seed cells into SeedEZ scaffold<br />
and place scaffolds into wells.<br />
Set perfusion rate on pump and begin<br />
perfusion<br />
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