PeloBiotech

www.pelobiotech.com
Ask for PD Collagenase!
The products below are defined by the total Wünsch activity per bottle, an assay that uses a peptide substrate to measure
the activity of the collagenase catalytic domain. This activity does not provide functional assessment of collagenase activity
but it is one of the most consistent and reliable activity measures used by many collagenase suppliers. The table below
provides ordering information and a cross reference chart to indicate which PD Collagenase product may meet your needs.
The Collagenase Gold product is similar to the PD Collagenase 800 but does not contain any supplemental purified
protease. This product enables users to use other proteases or to purchase from VC the same protease used in the PD
Collagenase products (BP Protease, catalog # 003-1000) for creation of a custom mixture.
Product Catalog # #/Quantity Cross Reference
PD Collagenase 100
PD Collagenase 800
Collagenase Gold
PB- 011-
1010
PB-011-
1050
PB-011-
1060
1 g
Worthington Type 1 or 2 Liberase TM or DH,
Blendzyme 1 or 2
1 g Liberase TL, Liberase DL, Liberase H I
1 g Human Islets
To isolate sensitive cells such as islets, high purity and fidelity collagenase are essential due to their critical role in enzymatic
degradation of the extracellular matrix without damaging the structural and functional integrity of the cells. Successful
human islet isolation requires the use of defined collagenase-protease enzyme mixtures, and intact class I and class II
collagenase should be used to ensure maximal islet recovery at the lowest dose of enzyme. This is why Collagenase Gold
is ideal for islet isolation. Thereby the use of these enzymes which are GMP and AOF can help with the best results. It has
been found that excess collagenase may potentially harm islets during the isolation process, emphasizing the need for
precise dosing and high purity enzymes to avoid detrimental effects on the isolated cells. Furthermore, studies have
demonstrated that islet mass, quality, and purity are determined by the efficacy of islet isolation, which depends on factors
such as organ procurement, collagenase digestion, and purification method. This highlights the critical role of high purity
collagenase in ensuring the quality and purity of isolated islets.
References
1. Breite AG, et al. Transplantation Proceedings 42 (2010); 2052-2054.
2. McCarthy RC, et al. Transplant Proc 40 (2008); 339-342.
3. Wünsch E and Hedrich H-G. Hoppe-Seyler’s Zeitschrift Physiol Chemie 333 (1963);149-151.
4. Mitchell WM and Harrington WF. Methods in Enzymology (1970) 635-642.
5. McCarthy RC, et al. Transplant 91 (2011) 137-145.
Scan the QR code to visit the VitaCyte website to get additional protocols and product
information.
6