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PeloBiotech

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www.pelobiotech.com<br />

Collagen based - Human/Rat/Bovine<br />

We offer bovine & rat type I collagen in different concentrations (3-8 mg).<br />

Human platelet lysate based photopolymerizable hydrogels<br />

Call us for more information (+49 89 517 286 59-0)<br />

3D ready Atelocollagen<br />

Stable three-dimensional culture (3D culture) can be performed easily just by adding your cells to atelocollagen. 3D Ready<br />

Atelocollagen is premixed with DMEM low or high glucose and can be used at once. You only need to warm the<br />

atelocollagen up with your cells.<br />

Atelocollagen is produced from Bovine dermis and contains collagen type I (95%) and type III and type IV (approx. 5%).<br />

You can use the hydrogels to culture cells within the collagen gel or on top of the hydrogel. You can easily set up cocultures<br />

by adding one cell type into the hydrogel and the second cell type will be<br />

cultured on top.<br />

Features:<br />

• Ready-to-use collagen solution for 3D cell culture<br />

• Premixed with DMEM, low or high glucose<br />

• Easy to handle: just mix with cells and warm up for gel-formation<br />

• Low cell sedimentation during gel formation<br />

3D Models Ready-to use<br />

Scilia Extragel Matrix Products<br />

Scilia Extragel Matrix is a reconstituted hydrogel formed by basement membrane components which are extracted from<br />

mouse tumor tissues. The hydrogel is rich in extracellular matrix proteins and is mainly composed of laminin, collagen IV<br />

and heparan sulfate proteoglycans. Various growth factors (EGF, PDGF, HGF, FGF-2, TGF-ß and IGF are also part of the<br />

hydrogel.<br />

Applications:<br />

Key Benefits:<br />

• 3D cell culture - High Safety (tested negative for LDEV)<br />

• Angiogenesis - Lot-to-Lot Consistency<br />

• Stem cell maintenance - High Compatibility<br />

• Stem cell differentiation - Ample Production<br />

• In vivo xenograft generation<br />

Products available as standard hydrogel (8-12 mg/ml), as high concentration (18-22 mg/ml), with reduced growth<br />

factors and hESC qualified. All media are also available phenol-red-free.<br />

Figure 2: Human bile duct organoids in Scilia<br />

Extragel Matrix. Organoids are imaged after<br />

being stained with DAPI (nucleus-blue), anti-<br />

ZO-1 (tight-junction protein) and Alexa Fluor<br />

647 Phalloidin (cytoskeleton protein F-catin).<br />

Pic: Beijing Megarobo Technologies<br />

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