PeloBiotech
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www.pelobiotech.com<br />
3D Scaffold-free<br />
3D CoSeedis<br />
The leading Swiss expert in Biostandardisation abc biopply ag has developed the innovative modular multi-purpose 3D<br />
Cell Co-Culture System 3D CoSeedis TM .<br />
Biostandardization respects clinical requirements already in the stage of molecular<br />
research and therefore can safe years in therapeutic development. With this unique and easy readout system a new standard<br />
in 3D Co-Culture is set. The novel scaffold-free 3D cell co-culture system consists of a unique conical agarose matrix array<br />
(CAMA) that allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent<br />
manner. The conically shaped microwells within the array allow precise determination of aggregate volume and cell growth.<br />
Furthermore, the modular composition of the 3D system allows distance co-cultures and, consequently, the standardization<br />
of protocols.<br />
Key Features<br />
Matrix and Modular Set-up<br />
• Contact or distance co-cultures (fully separable)<br />
• Serum, serum-reduced or serum-free conditions possible<br />
Permeable Matrix<br />
• Allows long term 3D culture of cell aggregates<br />
• Efficient use of physically separated feeder cells (distance co-culture)<br />
• Cultivation in standard or defined media<br />
Multi-purpose Applicability<br />
• Applicable to various cell types (see Applicable Cell Systems)<br />
• Supports the formation of aggregates of spheroidal and non-spheroidal cell types<br />
• Highly reproducible cell aggregates<br />
• Suitable for high-throughput applications<br />
Unique and easy Readout<br />
• Integrated method for histological analysis (H&E, IHC, ISH, etc.)<br />
3D CoSeedis is validated for the following applications:<br />
• 3D growth monitoring by volumetric analysis<br />
•<br />
Life/dead TM<br />
• Viability assays<br />
• HTS/HCS<br />
• Automated microscopy<br />
• Immunohistochemistry and -fluorescence<br />
• RNA, DNA and protein profiling<br />
• Extracellular vesicle production/profiling (e.g. EV-free mesenchymal stromal/stem cell co-cultures)<br />
• Whole-mount staining<br />
• Colony forming assay for tumorsphere formation and clonogenic survival<br />
Mass production of homogenous and uniform organoids/spheroids by using 3D CoSeedis TM<br />
One of the most important topics of 3D cell culture is a smooth and robust transition of 2D cell culture to 3D. This will help<br />
to create standardized workflows and protocols which are the fundament of reproducible and consistent results. abc biopply<br />
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