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www.pelobiotech.com<br />
As the stiffness of plastic culture vessels is far higher even than of bones we can imagine how artificial these cultures<br />
might be. A thin layer of a hydrogel can be helpful, but the solution will be a tuneable hydrogel.<br />
Cell Culture Reagents<br />
Insulin and Insulin-Related Products<br />
• rHu Insulin (cGMP): Recombinant human insulin used in cell culture, especially for studies involving glucose<br />
metabolism, diabetes, and insulin signaling pathways.<br />
• Bovine Insulin: Insulin derived from bovine sources, often used for similar applications as human insulin in cell<br />
culture.<br />
• Porcine Insulin: Insulin derived from porcine sources, employed in cell culture experiments related to diabetes,<br />
metabolism, and insulin signaling.<br />
• Insulin Solution, Human, Recombinant: A recombinant human insulin solution suitable for various cell culture<br />
and research applications.<br />
Cell Culture Solutions and Buffers: What about passaging?<br />
• Hanks Balanced Salt Solution without Ca2+ and Mg2+: A balanced salt solution used for cell culture<br />
applications, including the maintenance and washing of cells.<br />
• HBSS (100 ml): Hank's Balanced Salt Solution in a 100 ml format, ideal for smaller-scale cell culture experiments.<br />
• Dulbecco Phosphate-Buffered Saline, 1X (DPBS): A common buffer for cell culture applications, used for cell<br />
maintenance and experimental procedures.<br />
• PBS Buffer (1X): Phosphate-buffered saline (PBS) at a 1X concentration, widely used for cell washing and<br />
dilution in various biological assays.<br />
Chemicals and Additives<br />
• Heparin, Sodium Salt: Heparin is used as an anticoagulant in cell culture, especially when working with primary<br />
cells, stem cells, and coagulation studies.<br />
• Phenol Red (1ml) (minimum 10 vial order): Phenol red is a pH indicator often added to cell culture media to<br />
visually monitor pH changes.<br />
• Phenol Red (10ml): A larger quantity of phenol red for extended cell culture experiments.<br />
• Antibiotic Antimycotic Solution (100x): A high-concentration solution of antibiotics and antimycotics for<br />
preventing bacterial and fungal contamination in cell cultures.<br />
• Sodium Hydroxide (100 ml): Sodium hydroxide is used for adjusting pH levels in cell culture media.<br />
• L-Glutamine: An amino acid commonly added to cell culture media to promote cell growth.<br />
• 0.155 M NaCl: A saline solution used for various biological and cell culture applications.<br />
• Calcium Chloride Solution: Calcium chloride is employed in cell culture for inducing calcium-dependent<br />
processes in cells.<br />
• Lysis Buffer (5 ml): A buffer used for cell lysis and the release of cellular contents in various assays.<br />
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