PeloBiotech

www.pelobiotech.com
Tissue Dissociation
Tissue Dissociation is an extremely important early step in experiments using primary cells. This early step already
determines the quality of your cell culture and finally your results.
The main question is how to isolate cells in the best way and which criteria are the most important: Is it the cell
viability, the biomarker profile or just the cell number? Our partner VitaCyte offers only highly purified tissue
dissociation enzymes for basic research as well as for cell therapy.
CIzyme TM Tissue Dissociating Enzymes
Advantages of Purified Enzymes for Cell Isolation
Problems with crude or enriched collagenase:
Variable biochemical composition, reflects
heterogeneity of the bacterial culture supernatant
• Heterogenous molecular forms of
collagenase Lot qualification often
required
Collagenases and Proteases
Advantages of using purified enzymes:
Uniform collagenase composition, by HPLC and
specific CDA1 (CDA U/mg protein)
• Purified enzymes; minimal contamination
by other enzymes & endotoxin
• Enzyme formulation can be modified to
improve cell yield, viability, function
Collagenases and Proteases
Applications – Isolation of
• Hepatocytes
• Islet Cells
• Adipose Tissue Stem Cells
• Skin Fibroblast
• Skin Keratinocytes
• Cardiomyocytes
• Glioblastoma
NEW Products:
Recombinant Collagenase
Defined and enriched DE Collagenase products.
Collagenase is blended with purified neutral protease
at defined activities to provide a broad spectrum of
products with distinct collagenase activity.
Pic: High viable
cell yields: Get
consistent results
with our enzyme
formulations
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