PeloBiotech

www.pelobiotech.com
Further products of interest:
BloodStor®
The storage of stem cells from cord blood, peripheral blood, and bone marrow is crucial due to their potential therapeutic
uses in treating various diseases. Cord blood, in particular, contains hematopoietic stem cells (HSC) that can be used to treat
conditions such as leukemia, lymphoma, sickle cell disease, and other blood, bone, immune, and metabolic disorders.
Cord blood stem cells are being researched for their potential in regenerative medicine, where they may be used to induce
healing and regenerate cells to restore tissues. Furthermore, peripheral blood and bone marrow are also valuable sources
of stem cells for transplantation, with peripheral blood offering advantages such as easier and more common collection
compared to bone marrow. Exploratory analyses have indicated that peripheral-blood stem cells may reduce the risk of
graft failure, while bone marrow may reduce the risk of chronic graft-versus-host disease.
Proper storage of these stem cells is essential to maintain their viability and functionality for future therapeutic use. Umbilical
cord blood stem cells can be stored in cord blood banks for potential use in stem cell transplants, providing an "off-theshelf"
source of stem cells unlike bone marrow. The ability to store umbilical cord stem cells for future use presents a
significant clinical advantage. Moreover, the use of stem cells aspirated from bone marrow eliminates the need for the
creation of a second surgical site for harvesting autogenous bone, highlighting the importance of proper storage and
preservation of these cells.
ThawStar®
ThawStar® offers reproduceable thawing of cells this is important as studies have
shown that cryopreserved cells, such as mesenchymal stromal cells (MSCs), display
impaired immunomodulatory properties and reduced therapeutic potential
immediately after thawing. This impairment can lead to faster complement-mediated
elimination after exposure to blood, indicating a compromised functionality of the
cells. The cryopreservation process has been found to affect the viability and
metabolic function of cells, making them unsuitable for clinical use upon thawing. The alteration of the actin
cytoskeleton due to cryopreservation can also impact cell function and survival post-thawing.
The susceptibility of cryopreserved MSCs to T-cell mediated apoptosis and their altered biodistribution in vivo after
thawing further emphasize the detrimental effects of improper thawing on cell functionality. Moreover, the impact of
cryopreservation on cell migration ability and the alteration of the functional competency and survival of cells due to
freezing and thawing further highlight the importance of proper thawing procedures to maintain cell functionality. It
is evident that the processing, freezing, and thawing cycle can be detrimental to the cells, emphasizing the need for
optimized thawing protocols to preserve cell potency and therapeutic effect. Furthermore, the use of cryopreserved
products immediately after thawing has been associated with a higher failure rate compared to the use of fresh cells,
underscoring the critical nature of proper thawing procedures.
Cell Culture Supplements
Human and Bovine Serum Products:
• HSA Solution 25% (cGMP): Human Serum Albumin (HSA) is a versatile product used in various cell culture
applications. It serves as a stabilizer for enzymes, a blocking agent in Western blots, and a component of cell
culture media. Its high purity ensures consistent performance in diverse assays.
• Human AB Serum, Converted from Octaplas®: This converted human AB serum is crucial for culturing primary
cells, including hematopoietic cells and stem cells. It's xeno-free and virus-inactivated, making it suitable for a
wide range of cell culture applications.
• Human Serum Albumin (HSA) 25% CSS (cGMP): Closed System Solutions (CSS) of HSA is designed for
biomanufacturing applications, such as the production of vaccines, therapeutic proteins, and cell therapies.
• Bovine Serum Albumin (Fraction V): Bovine Serum Albumin (BSA) is a common protein supplement in cell
culture media. It stabilizes enzymes and acts as a carrier protein for hormones, fatty acids, and other
compounds. BSA is often used in immunocytochemistry, ELISA, and Western blotting.
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