PeloBiotech

www.pelobiotech.com
Media for Cryo-Preservation
The classical way to cryopreserve primary cells
is the usage of the culture medium plus 10-
20% FBS and 10% DMSO. After trypsination
of adherent growing cells, neutralization of
the used trypsin and centrifugation cells are
resuspended in the freezing medium (e.g.
Endothelial Cell Growth Medium + 10% FBS
+ 10% DMSO).
As alternative commercially available freezing
media can be used (Cryo-ROS, Cell freezing
medium and others) which contain 10%
DMSO. These media are available and tested
for different cell types e.g. blood cells,
hematopoietic stem cells, melanocytes,
macrophages, microglia and neural cells.
In recent years companies approached into the
market who are specialized in cryo-preservation and tissue storage media. One of our partners, BioLife Solutions, Seattle, has
developed a series of DMSO containing cryo media which are produced under GMP conditions and are suitable and tested
for a high number of cells. CryoStor CS 2, 5 and 10 is pre-formulated with USP grade DMSO of 2, 5 or 10%. The media is
based on the novel HypoThermosol formula.
Cryo-Preservation Media
CryoStor®
CryoStor® CS10 is a state-of-the-art cryopreservation medium designed to maximize postthaw
cell recovery and viability in extremely low temperatures ranging from -70°C to -
196°C. This ready-to-use solution is serum-free, animal component-free, and cGMPmanufactured,
ensuring a safe and protective environment for the cryopreservation of a
diverse array of sensitive cell and sample types. The formulation, containing 10% dimethyl
sulfoxide (DMSO) along with other essential ingredients, is meticulously crafted with USP-grade components to minimize
variability. This makes CryoStor® CS10 an ideal choice for e.g. preserving myeloma cell lines, human pluripotent stem
cells, blood-derived cells.
Available in various convenient formats, CryoStor® CS10 caters to the cryopreservation needs of B Cells, CHO Cells,
Hematopoietic Stem and Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem and Progenitor
Cells, Monocytes, Myeloma, NK Cells, Pluripotent Stem Cells, T Cells across different species, including Human, Mouse,
Non-Human Primate, and Rat. Whether applied in Cord Blood Banking, Epithelial Cell Biology, Immunology, or Stem Cell
Biology, CryoStor® CS10 stands out as an animal component-free, serum-free solution, ensuring the integrity and viability
of preserved cells. We also offer the same with 2% and 5% DMSO.
Cryo-Preservation by Vitrification
Vitrification is the solidification of a liquid without crystallization and growth of ice. It is an ultrafast
freezing method which is used for ES and iPS cells successfully. StemCell Keep is a DMSO-,
serum-, protein- and xeno-free cryopreservation medium for ES/iPS cells by vitrification.
Right: Vitrification is successful
Left: Recrystallization happened vitrification failed)
Procedure
1. Just add StemCell Keep to your cells after centrifugation and transfer the vial within 1 minute to liquid nitrogen.
2. Keep the vial for 1-2 minutes in liquid nitrogen and check the process.
3. Store cells in your liquid nitrogen tank.
34